Bcl-2抑制剂TW37联用紫杉醇对EC9706细胞增殖和凋亡的影响
Effects of Bcl-2 inhibitor TW37 combined with paclitaxel on proliferation and apoptosis of EC9706 cells

目的:观察Bcl-2抑制剂TW37和紫杉醇联用对食管癌EC9706细胞增殖、平板克隆形成及细胞凋亡的影响。方法:MTT法考察单用0.20～50.00 μmol/L紫杉醇、单用0.20～50.00 μmol/L TW37及联用后食管癌EC9706细胞的增殖抑制率; 平板克隆形成实验考察0.8 μmol/L紫杉醇、0.8 μmol/L TW37单用及联用对细胞克隆形成的影响; 流式细胞术检测单用及联用对细胞凋亡的影响。结果:MTT结果显示,Bcl-2抑制剂TW37联合紫杉醇作用可以抑制食管癌EC9706细胞增殖。细胞平板克隆形成实验证实,0.8 μmol/L 紫杉醇和0.8 μmol/L TW37都可以抑制克隆形成,2个化合物联用,能进一步抑制细胞集落的形成(P<0.001)。细胞凋亡检测结果显示,联用紫杉醇和TW37可以促进细胞凋亡(P<0.001)。结论:TW37联用紫杉醇可以抑制食管癌细胞增殖和克隆形成,促进细胞凋亡。
Aim: To observe the effects of Bcl-2 inhibitor TW37 combined with paclitaxel on cell proliferation, clonal formation and apoptosis of esophageal cancer EC9706 cells.Methods: MTT assay was used to examine the proliferation inhibition rate of cells EC9706 cells treated with 0.20-50.00 μmol/L paclitaxel alone or 0.20-50.00 μmol/L TW37 alone,or in combination. The plate clone formation experiment was used to investigate the clonal formation and flow cytometry was used to detect the apoptosis of EC9706 cells treated with 0.8 μmol/L paclitaxel or 0.8 μmol/L TW37 alone or in combination.Results: Bcl-2 inhibitor TW37 combined with paclitaxel could inhibit the proliferation of EC9706 cells.The plate colony formation experiment confirmed that both 0.8 μmol/L paclitaxel and 0.8 μmol/L TW37 could inhibit colony formation, and the combination of the two compounds could further inhibit the formation of cell colonies(P<0.001). The combination of paclitaxel and TW37 could promote apoptosis(P<0.001).Conclusion: TW37 combined with paclitaxel could inhibit the proliferation of esophageal cancer cells, inhibit cell clonal formation, and promote tumor cell apoptosis