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- 2016
复方续断三七提取物对家兔骨折愈合的促进作用及其机制
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Abstract:
摘要：目的 观察复方续断三七提取物(compound of radix dipsaci and pseudo-ginseng extract, CRPE)对家兔骨折愈合的促进作用，并探讨其机制。方法 采用家兔胫骨骨折模型，分成假手术组、模型组及CRPE 0.6g/kg、CRPE 1.2g/kg和CRPE 2.4g/kg组。观察CRPE对家兔骨骼愈合过程中骨痂形成时间、骨折愈合时间、血清中转化生长因子1(TGF-β1)、Ⅰ型胶原、Ⅱ型胶原、碱性磷酸酶、钙、磷、锌、铜含量的影响。采用Western blot和RT-PCR观察CRPE对家兔胫骨骨折组织中P-Smad2、P-Smad3、Smad7、PAI-1蛋白及mRNA的表达影响。结果 CRPE(1.2g/kg)组和CRPE(2.4g/kg)组能缩短骨痂形成时间和骨折愈合时间，显著升高骨折愈合过程中所需的TGF-β1、Ⅰ型胶原、Ⅱ型胶原、碱性磷酸酶、钙、锌、铜的含量；升高P-Smad2、P-Smad3和PAI-1的蛋白及mRNA的表达，降低Smad7蛋白及mRNA的表达(P<0.05)。结论 CRPE对家兔骨折预后有显著的促进作用，其机制可能与TGF-β1/Smad信号通路有关。
ABSTRACT: Objective To evaluate the effect and mechanism of the compound of radix dipsaci and pseudo-ginseng extract (CRPE) on bone fracture in rabbits. Methods The model of rabbit tibia fracture was used to detect the effects of CRPE on callus formation time and healing time. TGF-β1, collagen type Ⅰ, collagen type Ⅱ, alkaline phosphatase, Ca, P, Zn, Cu in rabbit serum were also measured. The protein and mRNA of P-Smad2, P-Smad3, Smad7 and PAI-1 were detected by RT-PCR and Western blot to evaluate the protective effect of CRPE. Results CRPE (1.2g/kg and 2.4g/kg) could significantly shorten callus formation time and healing time; and increase TGF-β1, collagen type Ⅰ, collagen type Ⅱ, alkaline phosphatase, Ca, P, Zn, Cu levels in rabbit serum. In addition, CRPE(1.2g/kg and 2.4g/kg) could increase P-Smad2, P-Smad3 and PAI-1 expressions and reduce the expression of Smad7 (P<0.05). Conclusion CRPE can improve the prognosis of bone fracture in rabbits and its mechanism might be related to TGF-β/Smad pathway
| [1] | 周燕，张纲，卓贤露，等. TGF-β在骨折愈合中的调控[J]. 免疫学杂志, 2012, 28(4):353-362. |
| [2] | 余志平，余高瞻，洪江. 骨折延迟愈合与血浆TGF-β1浓度的相关性分析[J]. 放射免疫学杂志, 2013, 26(6):850-851. |
| [3] | 吴兴明，邹永根，石睿. 伴有颅脑损伤对骨折患者血清中VEGF和TGF-β1水平影响研究[J]. 中国实验诊断学, 2015, 19(1):32-34. |
| [4] | SHI YG, MASSAGUE J. Mechanism of TGF-beta signaling from cell membrane to the nucleus[J]. Cell, 2003, 113(6):685-700. |
| [5] | HAYASHI H, ABDOLLAH S, QIU Y, et al. The MAD-related protein Smad7 associates with the TGF-beta receptor and functions as an antagonist of TGFbeta signaling[J]. Cell, 1997, 89(7):1165-1173. |
| [6] | 肖明锋. 对不同时期的骨折患者使用不同中药方剂进行治疗的效果探析[J]. 当代医药论丛, 2015, 13(6):33-34. |
| [7] | 董华，胡乃武，董清平. 骨痛仙胶囊对家兔骨折血清碱性磷酸酶、钙、磷影响的实验研究[J]. 中医药学报, 2003, 31(2):51-52. |
| [8] | 王伟卓，程一钊，郭雄，等. 大骨节病差异表达基因PAPSS2对成骨细胞矿化及碱性磷酸酶活性的影响[J]. 西安交通大学学报(医学版), 2014, 35(2):175-181. |
| [9] | 张奋耿，凌秋英，叶泽亮. 伤科接骨片对实验性家兔骨折模型血清微量元素含量的影响[J]. 中医正骨, 2008, 20(5):329-330. |
| [10] | STIPURSKY J, FRANCIS D, GOMES FC. Activation of MAPK/PI3K/SMAD pathways by TGF-β(1) controls differentiation of radial glia into astrocytes in vitro[J]. Dev Neurosci, 2012, 34(1):68-81. |
| [11] | MAO L, KAWAO N, TAMURA Y, et al. Plasminigen activator inhibitor-1 is involved in impaired bone repair associated with diabetes in female mice[J]. PLoS One, 2014, 9(3):e92686. |
| [12] | 李鹏飞，刘春莹，郭俊莹，等. 不同种类人参茎叶中皂苷成分的比较[J]. 安徽农业科学, 2010, 38(24):13077-13079. |
| [13] | 王磊，孙俊英. 血清TGF-β1和EGF检测对创伤性骨折愈合的影响[J]. 辽宁医学院学报, 2014, 35(4):28. |
