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-  2015 

抑制桩蛋白磷酸化对机械通气所致肺损伤的效应

DOI: doi:10.7507/1671-6205.2015090

Keywords: 机械通气, 肺损伤, 桩蛋白, 蛋白磷酸化

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Abstract:

目的研究抑制桩蛋白(Paxillin)磷酸化对机械通气相关性肺损伤的效应。 方法60只健康雄性SD大鼠随机分为正常对照组、保护性通气组、大潮气量通气组和PP2抑制剂组, 每组15只。正常对照组仅做气管切开; 保护性通气组进行保护性机械通气2 h, 大潮气量通气组进行大潮气量机械通气2 h; PP2抑制剂组通气前1 h腹腔注射酪氨酸蛋白激酶抑制剂PP2并进行大潮气量机械通气2 h。正常对照组在自然吸气2 h, 其余组在机械通气结束时处死, 光镜观察肺组织病理学改变并进行弥漫性肺泡损伤(DAD)评分; 测定髓过氧化物酶(MPO)活性; 计算肺湿/干重比值(W/D); 酶联免疫吸附试验(ELISA)检测肺泡灌洗液中肿瘤坏死因子(TNF-α)的水平; 伊文思蓝法检测肺血管通透性; 蛋白免疫印迹法检测磷酸化桩蛋白(p-Paxillin)和Paxillin表达情况; TUNEL法检测原位细胞凋亡。 结果大潮气量通气组和PP2抑制剂组的DAD评分、伊文思蓝渗出量、W/D、MPO及TNF-α差异均有统计学意义(P<0.05)。各组细胞原位凋亡率从高到低排序:大潮气量通气组>PP2抑制剂组>保护性通气组>正常对照组。p-Paxillin的表达量在大潮气量通气组中最高, 与其他组比较差异有统计学意义(P<0.05)。保护性通气组、大潮气量通气组和PP2抑制剂组间Paxillin的表达量差异无统计学意义(P>0.05)。 结论抑制Paxillin磷酸化可以明显改善机械通气相关性肺损伤

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