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-  2017 

3-D 成球培养 MSCs 的研究进展及临床应用前景

DOI: doi:10.7507/1002-1892.201612056

Keywords: 3-D 培养, 成球培养, MSCs, 干细胞疗法, 修复重建

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Abstract:

目的 总结近年来 3-D 成球培养 MSCs 的研究进展及临床应用前景。 方法 广泛查阅国内外 3-D 成球培养 MSCs 的相关研究,重点关注 3-D 培养模式下 MSCs 细胞球的形成机制,3-D 培养的 MSCs 细胞球与传统贴壁培养的 MSCs 在生物功能上存在的差异,以及造成这些差异的生物学机制,探讨 MSCs 细胞球的临床应用前景。 结果 与传统贴壁培养的 MSCs 相比,3-D 培养的 MSCs 细胞球在抗凋亡、多向分化、旁分泌、抗炎等多项生物功能上明显增强,其机制与细胞骨架的改变、细胞接触的增加和低氧微环境的刺激有关,在动物实验中对于难愈性创面的修复、缺血组织的修复、组织重塑具有显著治疗效果,有广阔临床应用前景。 结论 3-D 培养的 MSCs 细胞球较传统贴壁培养的 MSCs 具有更强的生物功能和治疗效果,可成为临床上优化干细胞疗法、提高其治疗效果的一种手段

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