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-  2016 

犬肩袖损伤愈合模型的研究

DOI: doi:10.7507/1002-1892.20160093

Keywords: 肩袖损伤, 动物模型, 半腱肌扩张部, 损伤修复, 制动,

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Abstract:

目的比较研究不同肩袖损伤类型以及采用不同方法修复后的肩袖愈合差异,探讨能准确模拟临床肩袖损伤修复术后恢复进程的动物模型。 方法取12只成年雄性比格犬,体质量10~15 kg,根据处理方法不同随机分为3组(n=4),分别制备急性肩袖损伤+Mason-Allen缝合修复(A组)、巨大肩袖损伤+Mason-Allen缝合修复(B组)及巨大肩袖损伤+Mason-Allen缝合并自体半腱肌扩张部修复(C组)动物模型;3组修复后均外固定架制动。术后观察各组动物一般情况;于术后6周取材大体观察冈下肌肌腱末端愈合情况,生物力学测试极限负荷,组织学观察肌腱细胞及纤维改变,比较肩袖修复程度。 结果术后各组动物均存活至实验完成,切口均愈合良好,无感染发生。大体观察示,A组冈下肌肌腱末端瘢痕组织明显多于正常肌腱组织;B组冈下肌肌腱末端未见明显肌腱组织;C组冈下肌肌腱虽然部分覆盖瘢痕组织,但仍可观察到肌腱及其大致走向。A、B、C组极限负荷分别为(223.75±24.28)、(159.25±34.87)、(233.25±14.24)N,B组显著低于A、C组(P<0.05),A、C组间比较差异无统计学意义(P>0.05)。组织学观察示,A组肌腱纤维排列大致正常;B组肌腱纤维排列较紊乱,并且肌腱细胞明显少于A组;C组肌腱纤维排列整齐,且肌腱细胞多于B组。 结论Mason-Allen缝合联合自体半腱肌扩张部修复犬巨大肩袖损伤能获良好修复效果;制动模型可较好模拟临床肩袖损伤愈合过程,可作为相关研究的理想动物模型

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