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-  2018 

基于毒性表型与微卫星标记的云南省条锈病菌群体遗传分析
Genetic analysis of Puccinia striiformis f. sp. tritici in Yunnan Province based on virulence phenotypes and simple sequence repeats

DOI: 10.13802/j.cnki.zwbhxb.2018.2018908

Keywords: 条锈菌 群体结构 遗传分化
Puccinia striiformis f. sp. tritici population structure genetic differentiation

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Abstract:

为明确云南省条锈菌群体结构和遗传多样性水平,利用19个中国鉴别寄主和11对微卫星(simple sequence repeat,SSR)标记引物对采自云南省大理、曲靖、文山、昭通4个市的88个菌系进行了毒性表型分析和分子基因型分析。毒性表型分析结果表明,云南省条锈菌群体Nei’s基因多样性指数为0.17,Kosman指数为0.22,云南省条锈菌群体毒性结构复杂;菌系以Hybrid 46类群(77.3%)和贵农22类群(17.0%)为主,并且均不能侵染鉴别寄主中四和Triticum spelta album。分子基因型分析结果表明,云南省条锈菌整体上香农信息指数为0.63,分子遗传多样性比较丰富;大理市菌系香农信息指数为0.64,分子遗传多样性最丰富;文山市菌系香农信息指数为0.35,分子遗传多样性最低。聚类分析结果表明,云南省西部大理市菌系明显不同于云南省东部曲靖市、昭通市、文山市的菌系,昭通市菌系和曲靖市菌系毒性结构和基因型结构基本相同,表明云南省东、西部条锈菌群体遗传分化程度较高,是2个相对独立的流行区域。
To investigate population structure and genetic diversity of Puccinia striiformis f. sp. tritici in Yunnan Province, 88 single-pustule isolates collected from Yunnan Province(Dali City, Qujing City, Wenshan City and Zhaotong City) were tested against 19 Chinese differentials and genotyped at 11 simple-sequence repeat loci. Virulence phenotype analysis showed that Nei’s gene diversity index and Kosman index of Yunnan stripe rust were 0.17 and 0.22, respectively. The population structure of stripe rust in Yunnan Province was complex. The dominant isolates belonged to Hybrid 46 pathogenic group(77.3%) and Guinong 22 pathogenic group(17.0%), and none of them could infect Zhong 4 and Triticum spelta album. Molecular genotype analysis showed that the overall Shannon information index of stripe rust in Yunnan Province was 0.63, suggesting that the genetic diversity level of stripe rust in Yunnan Province was relatively high. The molecular genetic diversity of stripe rust in Dali City was the most abundant, with a Shannon information index of 0.64. The molecular genetic diversity of stripe rust in Wenshan City was the lowest, with a Shannon information index of 0.35. Cluster analysis showed that stripe rust in Dali City of western Yunnan Province was significantly different from those in Qujing City, Zhaotong City and Wenshan City in eastern Yunnan Province. The virulence and genotypic structure of stripe rust in Zhaotong City and Qujing City was almost the same. The degree of genetic differentiation of the pathogen between eastern and western Yunnan Province was high, indicating that they were two relatively independent regions in Yunnan epidemic zone.

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