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-  2018 

4-羟基-3-甲氧基肉桂酸乙酯的生物活性及其除草作用机制
The biological activity of 4-hydroxy-3-methoxycinnamic acid ethyl ester and its herbicidal mechanism

DOI: 10.13802/j.cnki.zwbhxb.2018.2016210

Keywords: 4-羟基-3-甲氧基肉桂酸乙酯 生物活性 作用机制
4-hydroxy-3-methoxycinnamic acid ethyl ester biological activity mechanism of action

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Abstract:

为明确以天然产物阿魏酸为母体进行化学结构改造得到的化合物4-羟基-3-甲氧基肉桂酸乙酯的生物活性及其除草机制,利用茎叶喷雾法和小杯法测定其对马唐和反枝苋的生物活性,基于生理生化指标及功能基因表达量对其除草作用机制进行研究。结果表明:4-羟基-3-甲氧基肉桂酸乙酯对马唐茎叶鲜重抑制中浓度IC50为46.27 mg/L;对反枝苋胚根和芽的IC50分别为129.94 mg/L和147.33 mg/L,且在500 mg/L浓度处理时可抑制反枝苋种子萌发;对拟南芥根长的IC50为43.82 mg/L,在160 mg/L浓度处理时拟南芥几乎停止生长、叶片发黄、根毛堆积。该化合物以1 000 mg/L浓度处理马唐叶片2、3、8 h后,电导率较对照组分别增加了22.70%、18.46%和25.62%;1 000 mg/L浓度处理下叶绿素a/b为2.43,较对照的3.62显著下降;处理后叶表微观结构观察发现化合物浓度越大,植物叶片表皮毛倒伏和褶皱越严重;拟南芥AT4G04820基因表达量显著下降,在处理45 min时下降最显著,与对照组相差42.52倍,微管蛋白含量下降,造成微管解离,从而抑制植物生长。表明4-羟基-3-甲氧基肉桂酸乙酯具有较强的除草活性,可能通过破坏细胞结构、抑制光合作用及阻止根生长等途径使杂草生长受到抑制甚至死亡。
In order to clarify the biological activity and weeding mechanism of 4-hydroxy-3-methoxy cinnamic acid ethyl ester, which was obtained by modifying a natural product of fumalic acid in the previous research, its bioactivity to two weeds, Digitaria sanguinalis and Amaranthus retroflexus, were measured by using the methods of foliar treatment and small glass, and its herbicidal mechanism was studied by using the physiological and biochemical indicators and functional gene expression. The results showed that 4-hydroxy-3-methoxy cinnamic acid ethyl ester had a median inhibitory concentration (IC50) value of 46.27 mg/L on the fresh weight of D. sanguinalis stems and leaves, with an IC50 value of 129.94 mg/L and 147.33 mg/L on radical and bud of A. retroflexus, respectively, and the germination could be inhibited at a concentration of 500 mg/L, also with an IC50 value of 43.82 mg/L to the root of Arabidopsis thaliana and could completely inhibit the growth, making the leaves turn yellow and the root hairs accumulated at 160 mg/L. After being treated at 1 000 mg/L for 2, 3 and 8 h, the electric conductivities of D. sanguinalis leaves increased by 22.70%, 18.46% and 25.62%, respectively, compared with the control, and the chlorophyll a/b contents significantly decreased from 3.62 to 2.43. The epidermal hairs of D. sanguinalis leaves sprayed by the compound displayed lodging and corrugating in proportion to the concentration. The expression quantity of gene AT4G04820 of A. thaliana declined dramatically, with the greatest decrements of 42.52-fold at 45 min, and significant decrease in tubulin amount resulted in microtubule dissociation and finally growth inhibition. The results indicated that 4-hydroxy-3-methoxy cinnamic acid ethyl ester had a strong herbicidal activity, probably due to the damage of cell structure and the inhibition on photosynthesis and root growth of plants.

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