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- 2015
Ti-HA-BaTiO3生物复合材料的制备与性能
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Abstract:
为进一步提高骨修复材料的骨诱导性能, 引入了BaTiO3压电涂层.首先, 通过等离子喷涂法在Ti基体上制备羟基磷灰石(HA)、Ti-HA和Ti-HA-BaTiO3这3种生物涂层;然后, 采用正交试验设计优化了喷涂工艺参数, 用SEM/EDS对涂层的形貌和成分进行了表征, 并通过划痕试验测试了涂层与基体的结合强度;最后, 进行了细胞培养试验, 用CCK-8法对细胞毒性进行了评价, 并在SEM下观察了细胞的粘附形态.结果显示:细胞带有伪足, 呈梭形和不规则多边形, 且粘附在Ti-HA-BaTiO3复合涂层表面;细胞伸展形态良好, 涂层的细胞毒性等级不高于1级.可见, Ti-HA-BaTiO3复合材料可以作为临床植入材料应用. In order to further improve the bone inductive properties of bone repair materials, BaTiO3piezoelectric coating was introduced.First, three kinds of biological coatings of hydroxyapatite (HA), Ti-HA and Ti-HA-BaTiO3 were prepared on Ti substrates by plasma spraying method. Then, the spray process parameters were optimized by orthogonal test design, the morphology and composition of coatings were characterized by SEM/EDS, and the bonding strength between coating and substrate was tested by scratch test. Finally, the cell culture test was conducted, the cytotoxicity was evaluated by CCK-8 method, and the adhesion morphology of cells was observed under SEM. The results show that cells have pseudopodia, present fusiform and irregular polygons, and adhere to the surface of Ti-HA-BaTiO3 composite coating; cells spread in good shape, the cytotoxicity level of coating is no higher than Level 1.It follows that Ti-HA-BaTiO3bio-composites can be used as clinical implant materials. 国家自然科学基金(51455055);新疆维吾尔自治区青年科学基金(2013211B59);新疆重大疾病医学重点实验室开放课题(SKLIB-XJMDR-2014-Y1)
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