运动调节APP/PS1小鼠海马CaMKⅡα、AMPAR活性，增加突触可塑性
Aerobic Exercise Increases Synaptic Plasticity by Regulating the Activity of AMPA Receptor and CaMKⅡα in the Hippocampus of APP/PS1 Mice

摘要：目的：探讨有氧运动对8月龄APP/PS1小鼠海马CaMKⅡα和AMPAR的作用，为有氧运动促进神经功能的恢复提供一定理论依据。方法：清洁级4月龄APP/PS1、C57BL/6J小鼠各30只，随机分为安静对照组和有氧运动组，每组15只，运动组小鼠进行16周跑台运动。跳台回避实验检测小鼠行为学变化；透射电镜观察海马突触数目的变化；western blot检测海马AMPAR和CaMKⅡα的表达。结果：16周有氧运动可明显提高APP/PS1小鼠的行为学表现，增加海马突触的数目，增加海马CaMKⅡα蛋白表达，上调AMPAR的GluR1亚基的活性。结论：规律有氧运动通过增加8月龄APP/PS1小鼠海马CaMKⅡα含量，上调AMPAR 的GluR1亚基活性，增加海马突触可塑性，是运动改善其行为学能力的分子机制之一。
Abstract: Objective: To investigate the effect of aerobic exercise on Ca2+/calmodulin-dependent protein kinase II α (CaMKⅡα ) and AMPA receptor in the hippocampus of 8 months old APP/PS1 mice in order to provide a theoretical basis for promoting the recovery of neural function by aerobic exercise. Methods: Thirty APP/PS1 transgenic mice (Clean grade, 4 months) and thirty C57BL/6J mice (Clean grade, 4 months) were randomly divided into 16 weeks aerobic exercise group and sedentary group, 15 mice respectively. Step-down avoidance test was used to evaluate their behavioral changes, transmission electron microscope was used to observe the changes in the number of hippocampal synapses and western blotting was used to test the expression of AMPAR and CaMKⅡα in the hippocampus of mice. Results: Sixteen weeks aerobic exercise can significantly improve the behavioral manifestations of APP/PS1 mice, increase the number of synapses and the expression of CaMKⅡα in the hippocampus, and raise the activity of AMPAR GluR1. Conclusions: Regular aerobic exercise can increase the activity of AMPAR GluR1 and increase synaptic plasticity in the hippocampus by increasing the content of CaMK alpha in the hippocampus of APP/PS1 mice aged 8 months old. This might be one of the molecular mechanisms of exercise to improve their behavior ability