|
|
- 2016
健康儿童及成人唾液微生物多样性研究
|
Abstract:
摘要 目的:16S rRNA高通量测序技术分析口腔健康儿童与成人唾液微生物多样性的差异。方法:选择11位受检者(5例口腔健康成人(AJ组),6例口腔健康儿童(CJ组))的唾液样本进行细菌DNA提取,后利用Illumina MiSeq测序平台对11例样本DNA的16S rRNA V4-V5区进行测序,并分析两组唾液样本间菌群多样性。结果:11个样本总获得 474119 条序列,共9223OTUs,归属于26个门,374个属;PcoA检测显示两组微生物群落结构有明显差异;T-检验显示两组间差异菌属为:纤毛菌属(Leptotrichia)、卟啉单胞菌属(Porphyromona)、密螺旋体属(Treponema)、孪生球菌属(Gemella)、颗粒链菌属(Granulicatella);其中,卟啉单胞菌属、密螺旋体属、纤毛菌属在AJ组的相对丰度高于CJ组,孪生球菌属、颗粒链菌属在CJ组相对丰度高于AJ组。结论:健康成人与健康儿童口腔唾液微生物群落结构在种类上相似,而在丰度上存在差异,差异提示:健康成人较健康儿童更倾向患牙周病;健康儿童较健康成人更倾向于患龋病
| [1] | Gomar-Vercher S, Cabrera-Rubio R, A Mria, et al. Relationship of children's salivary microbiota with their caries status: a pyrosequencing study [J]. Clin Oral Invest, 2014, 18(9)∶2087-2094 |
| [2] | Jaqathrakshakan SN,Sethumadhava RJ, Mehta DT, et al. 16S rRNA gene-based metagenomic analysis identifies a novel bacterial co-prevalence pattern in dental caries [J]. Eur J Dent,2015,9(1)∶127-132 |
| [3] | Struzcka I. The Oral Microbiome in Dental Caries [J]. Polish Journal Of Microbiology,2014,63(2)∶127-135 |
| [4] | Wade W.G, Has the use of molecular methods for the characterization of the human oral microbiome changed our understanding of the role of bacteria in the pathogenesis of periodontal disease [J]. J Clin Peridontol,2011,38(s11)∶7-16 |
| [5] | Jiang W, Zhang J, Chen H. Pyrosequencing Analysis of Oral Microbiota in Children with Severe Early Childhood Dental Caries [J]. Curr Microbiol,2013,67(5)∶537-542 |
| [6] | 周学东,施文元.口腔微生态学[M].北京:人民卫生出版社,2013 |
| [7] | 周学东,肖丽英,肖晓蓉.实用口腔微生物学与技术[M].北京:人民卫生出版社,2009 |
| [8] | Giannobile WV, McDevitt JT, Niedbala RS, et al. Translational and clinical applications of salivary diagnostics [J]. Advances In Dental Research, 2013, 23(4)∶375-380 |
| [9] | Ling ZX, Liu X, Wang YZ, et al. Pyrosequencing Analysis of the Salivary Microbiota of Healthy Chinese Children and Adults [J]. Microbial Ecology, 2012, 65(2)∶487-495 |
| [10] | Ge X,Rodriguez R,Trinh M, et al. Oral microbiome of deep and shallow dental pockets in chronic periodontiitis [J]. Plos One,2013,8(6)∶e65520 |
| [11] | Gross EL, Ley EJ, Gasparovich SR, et al. Bacterial 16S sequence analysis of severe caries in young permanent teeth [J]. J.Clin.Microbiol, 2011, 48(11)∶4121-4128 |
| [12] | Chen H,Jiang W. Application of high-throughput sequencing in understanding human oral microbiome related with health and disease [J]. Front Microbiol,2014,5∶508 |
| [13] | Xu P, Gunsolley J. Application of metagenomics in understanding oral health and disease [J].Virulence,2014,5(3)∶424-432 |
| [14] | Scher JU, Ubeda C, Equinda M, et al. Periodontal disease and the oral microbiota in new-onset rheumatoid arthritis [J]. Arthritis And Rheumatism,2012,64(10)∶3083-3094 |
| [15] | Amato K.R, Yeoman C.J, Kent A, et al. Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes [J]. The ISME journal,2013,7(7)∶1344-1353 |
| [16] | 袁杰,王少果,周建业,等.慢性牙周炎唾液牙龈卟啉单胞菌的定量PCR检测[J].口腔医学研究,2015,31(2)∶133-135 |
| [17] | He J, Li Y, Cao Y, et al. The oral microbiome diversity and its relation to human diseases [J]. Folia Microbiologica, 2015, 60(1)∶69-80 |
| [18] | Belstr m D, Fiehn NE, Nielsen CH, et al. Differences in bacterial saliva profile between periodontitis patients and a control cohort [J]. J Clinl Periodontol,2014,41(2)∶104-112 |
