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- 2018
N-Ac-L-Leu-PEI/微小RNA-34a复合物对大鼠颅骨缺损成骨的影响
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Abstract:
摘要 目的:将N-Ac-L-Leu-PEI/微小RNA(microRNA,miR)-34a复合物用于大鼠颅骨缺损模型,探讨miR-34a对骨缺损区成骨的影响。方法:Wistar雄性大鼠32只,制备标准大鼠颅骨缺损模型,随机分为4组,分别于缺损区覆盖载有磷酸盐缓冲液 (phosphate buffered salin, PBS)、N-Ac-L-Leu-PEI、N-Ac-L-Leu-PEI/miR-34a复合物、N-Ac-L-Leu-PEI/miR-34a NC复合物的口腔生物膜,饲养10周后处死大鼠,通过锥形束CT (cone beam computerized tomography,CBCT)、微型CT (microcomputed tomography,micro-CT)、反转录-聚合酶链反应(reverse transcription-polymerase chain reaction, RT-PCR)等方法检测大鼠颅骨缺损区新骨形成情况。结果:N-Ac-L-Leu-PEI/miR-34a复合物组缺损区骨新生最为明显,新生骨量与总组织量之比 (bone volume / total volum, BV/TV)、骨小梁厚度 (trabecular thickness, Tb.Th)值均高于其它组,成骨标志性基因Runt相关转录因子2 ( runt-related transcription factor 2, Runx2)、Osterix转录因子 (serine protease 7, SP7)及Ⅰ型胶原 (Collagen Ⅰ, ColⅠ)的mRNA表达显著上调。结论:对实验性大鼠颅骨缺损模型,N-Ac-L-Leu-PEI/miR-34a具有促进缺损区成骨的作用
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