|
|
- 2015
茜素红与阿利新蓝双染色法对小鼠下颌骨发育的整体观察
|
Abstract:
摘要 目的:探索用茜素红/阿利新蓝双染色法观察小鼠下颌骨的整体骨架发育。方法:应用不同条件的茜素红/阿利新蓝染色液对E15.5、E16.5、 E18.5和P90的小鼠进行整体骨架双染色,观察小鼠下颌骨的整体发育情况,重点关注Meckel's软骨和髁突软骨发育。结果:骨和软骨能被分别染成红色和蓝色,在体视显微镜下可清楚地观察到下颌骨的整体发育形态。Meckel's软骨与周围的骨组织在组织学上和空间上无连续性。髁突软骨独立于Meckel's软骨,与下颌骨骨膜紧密相连。结论:应用不同条件的茜素红/阿利新蓝整体骨架双染色法成功观察了小鼠下颌骨的整体发育过程。Meckel's软骨可能不直接参与下颌骨的骨化,髁突可能是来源于下颌骨末端骨膜间质细胞。[关键词] 阿利新蓝 茜素红 双染色法 下颌骨发育 整体观
| [1] | Bhaskar SN, Weinmann JP, Schour I. Role of Meckel's cartilage in the development and growth of the rat mandible [J]. J Dent Res, 1953, 32(3)∶398-410 |
| [2] | Ramaesh T, Bard JB. The growth and morphogenesis of the early mouse mandible: a quantitative analysis [J]. J Anat, 2003, 203(2)∶213-222[9] Depew MJ, Simpson CA, Morasso M, et al. Reassessing the Dlx code: the genetic regulation of branchial arch skeletal pattern and development [J]. J Anat, 2005, 207(5)∶501-561 |
| [3] | Nakao K, Okubo Y, Yasoda A, et al. The effects of C-type natriuretic peptide on craniofacial skeletogenesis [J]. J Dent Res, 2013, 92(1)∶58-64 |
| [4] | Chai Y, Maxson RE, Jr. Recent advances in craniofacial morphogenesis [J]. Dev Dyn, 2006, 235(9)∶2353-2375 |
| [5] | Knight RD, Schilling TF. Cranial neural crest and development of the head skeleton [J]. Adv Exp Med Biol, 2006, 589∶120-133 |
| [6] | Tomo S, Ogita M, Tomo I. Development of mandibular cartilages in the rat [J]. Anat Rec, 1997,249(2)∶233-239 |
| [7] | Ishizeki K, Saito H, Shinagawa T, et al. Histochemical and immunohistochemical analysis of the mechanism of calcification of Meckel's cartilage during mandible development in rodents [J]. J Anat, 1999, 194 ( Pt 2)∶265-277 |
| [8] | Yang RT, Zhang C, Liu Y, et al. Autophagy prior to chondrocyte cell death during the degeneration of Meckel's cartilage [J]. Anat Rec (Hoboken), 2012, 295(5)∶734-741 |
| [9] | Vinkka-Puhakka H, Thesleff I. Initiation of secondary cartilage in the mandible of the Syrian hamster in the absence of muscle function [J]. Arch Oral Biol, 1993,38(1)∶49-54 |
| [10] | Chai Y, Jiang X, Ito Y, et al. Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis [J]. Development, 2000,127(8)∶1671-1679 |
| [11] | Kamiya Y, Chen J, Xu M, et al. Increased mandibular condylar growth in mice with estrogen receptor beta deficiency [J]. J Bone Miner Res, 2013, 28(5)∶1127-1134 |
| [12] | Shibata S, Fukada K, Suzuki S, et al. In situ hybridization and immunohistochemistry of bone sialoprotein and secreted phosphoprotein 1 (osteopontin) in the developing mouse mandibular condylar cartilage compared with limb bud cartilage [J]. J Anat, 2002, 200(Pt 3)∶309-320 |
| [13] | 张燕丽,张翠珍,彭刚,等.斑马鱼颅骨骨组织发育特征的研究[J].口腔医学研究,2014,30(2)∶97-99 |
| [14] | Wyganowska-Swiatkowska M, Przystanska A. The Meckel's cartilage in human embryonic and early fetal periods [J]. Anat Sci Int, 2011, 86(2)∶98-107 |
| [15] | 华先明,程祥荣.下颌髁突软骨的结构和在拉伸/剪切负荷作用下的生物机械性能[J].口腔医学研究,2014,30(2)∶177-179 |
| [16] | Alexander C, Piloto S, Le Pabic P, et al. Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton [J]. PLoS Genet, 2014,10(7)∶e1004479 |
