|
|
- 2018
无血清培养下富血小板纤维蛋白提取液对牙髓干细胞增殖分化的影响
|
Abstract:
摘要 目的:在无血清培养条件下,使用富血小板纤维蛋白提取液(platelet-rich fibrin extract,PRFe)对人牙髓干细胞(human dental pulp stem cells,hDPSCs)增殖及分化的影响。方法:从健康志愿者的阻生齿中分离培养hDPSCs,经细胞形态和流式细胞技术对其鉴定。实验组中使用含PRFe不含胎牛血清(fetal bovine serum,FBS)的α-最低必须培养基(α-minimal essential medium,α-MEM),对照组中使用含10%FBS的α-MEM。噻唑蓝(methyl thiazol tetrazolium,MTT)法检测培养1~7 d的细胞增殖情况。成骨能力的测定采用碱性磷酸酶染色,实时荧光定量聚合酶链式反应(real time quantitive-polymerase chain reaction,RT-PCR)检测碱性磷酸酶(alkaline phosphatase,ALP)、骨钙蛋白(osteocalcin,OCN)、骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)和runt相关转录因子2(runt-related transcription factor-2,RUNX-2)的表达。结果: hDPSCs高表达CD44、CD90和CD105,低表达CD34和CD45。两种培养基培养细胞形态相似,实验组的细胞形态更为纤长。随着诱导时间的增加,两组的成骨分化能力均上调(P<0.05),实验组成骨分化能力强于对照组(P<0.05)。结论: 适宜浓度PRFe能够维持hDPSCs增殖分化
| [1] | Trubiani O, Diomede F. Xeno-free. Culture of human periodontal ligament stem cells [J]. Methods Mol Biol, 2015, 128(3):87-92 |
| [2] | Choukroun J, Adda F, Schoeffler C, et al. Une opportunite enparo-implantologie: Le PRF [J]. Implantodontie, 2001, 4(2):55-62 |
| [3] | Kim JH, Woo SM, Choi NK, et al. Effect of platelet-rich fibrin on odontoblastic differentiation in human dental pulp cells exposed to lipopolysaccharide [J]. J Endod, 2017, 43(3):433-438 |
| [4] | 董凯,柳忠豪,张晓洁,等.富血小板纤维蛋白提取液对MC3T3-E1细胞影响的实验研究[J].中华口腔医学杂志,2013,48(5):288-293 |
| [5] | 杨雪超,吕孝帅.CD146在人牙髓干细胞中表达的研究[J].口腔医学研究,2016,32(2):122-125 |
| [6] | 王冉旭,潘爽,何丽娜,等.人牙髓干细胞在不同HA含量的PLGA/HA复合支架材料上的粘附研究[J].口腔医学研究,2015,31(12):1171-1173 |
| [7] | 张晓洁,许胜,孟维艳,等.富血小板纤维蛋白提取液对钛表面成骨细胞黏附、增殖及分化的影响[J].中华口腔医学杂志,2017,52(1):48-52 |
| [8] | Wang ZS, Feng ZH, Wu GF, et al. The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering [J]. Sci Rep, 2016, 6:28126 |
| [9] | Abd M, Wang X, Miusi S, et al. Injectable-platelet rich fibrin using the low speed centrifugation concept improves cartilage regeneration when compared to platelet-rich plasma [J]. Platelets, 2017:1-9 |
| [10] | Yee M, Kim A, Alpagot T, et al. Porphyromonas gingivalis stimulates IL-18 secretion in human monocytic THP-1 cells [J]. Microbes Infect, 2012, 14(9):684-689 |
