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- 2018
布比他丁促进大鼠骨髓间充质干细胞CXC型趋化因子受体4膜向分布及迁移能力的研究
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Abstract:
摘要 目的:研究药物布比他丁(Blebbistatin)能否调节大鼠骨髓间充质干细胞( bone marrow mesenchymal stem cells,BMSCs)表面CXC型趋化因子受体4 (CXC chemokine receptor type 4,CXCR4)向胞膜分布,从而促进大鼠BMSCs的迁移。方法:分离培养SD大鼠BMSCs,Blebbistatin处理细胞15、30、45、60 min,设置对照组;流式细胞术测定细胞表面CXCR4表达;Transwell迁移实验检测细胞迁移能力;测定成骨诱导前期培养基碱性磷酸酶(alkaline phosphatase,ALP)活性,待诱导完成进行茜素红染色。结果: 处理组细胞表面CXCR4表达率明显升高(P<0.01),且呈时间依赖性;15、30和60 min组细胞迁移效率升高(P<0.01)。成骨诱导期间各组间ALP活性比较差异无统计学意义,诱导至21 d时各组均形成大量钙化结节。结论: Blebbistatin能促进BMSCs表面CXCR4向胞膜分布,并能提高BMSCs的迁移效率
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