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-  2016 

MicroRNA-451对大鼠心肌缺血/再灌注损伤的影响 Effects of MicroRNA-451 on Myocardial Ischemia/Reperfusion Injury in Rats

Keywords: microRNA-451,心肌缺血,再灌注,高迁移率族蛋白1

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Abstract:

目的:探讨MicroRNA(miR)-451在大鼠心肌缺血/再灌注(I/R)损伤中的作用及其作用机制。方法:将70只SD大鼠随机分为5组:假手术组(SO)、缺血再灌注组(I/R)、腺病毒空载体组(I/R+Ad-GFP)、miR-451上调组(I/R+Ad-miR-451)、miR-451下调组(I/R+Ad-asmiR-451)。心肌注射病毒液(1×1010 pfu/只)或PBS液(150μl/只)后3d建立缺血30min再灌注24h的心肌I/R模型。应用TTC+伊文思蓝双染法测定各组心肌梗死面积百分比,TUNEL法检测凋亡率,Western Blot法测定HMGB1和Caspase 3活化片段含量,应用试剂盒检测血清CK、LDH含量以及心肌组织SOD、MDA含量。结果:再灌注24h后,与SO组相比,I/R组和I/R+Ad-GFP组血清CK、LDH含量明显升高,心肌组织SOD活性降低、MDA含量升高,心肌组织HMGB1及Caspase-3活化片段蛋白含量显著上升,心肌细胞凋亡指数明显增加(以上P<0.05);与I/R组、I/R+Ad-GFP组相比,I/R+Ad-miR-451组血清CK、LDH含量显著下降,心肌组织SOD活性上升、MDA含量下降,心肌组织HMGB1和Caspase-3活化片段蛋白含量下降,心肌梗死面积百分比降低,心肌细胞凋亡指数降低(以上P<0.05);与I/R组、I/R+Ad-GFP组相比,I/R+Ad-asmiR-451组血清CK、LDH含量无明显上升,心肌组织MDA含量及SOD活性无明显变化,心肌组织HMGB1表达含量无明显上升,心肌梗死面积百分比及心肌细胞凋亡指数无显著升高(以上P>0.05),Caspase-3活化片段蛋白含量上升(P<0.05)。结论:MicroRNA-451通过调控HMGB1的表达,减轻凋亡和氧化应激进而减轻心肌I/R损伤

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