|
|
- 2015
豆状带绦虫四川株的生物学研究
|
Abstract:
中文摘要： 目的 对豆状带绦虫四川株的生物学特性进行深入研究,为豆状囊尾蚴病的防控提供基础数据.方法用豆状带绦虫虫卵经口感染兔后,于第60天剖杀采集豆状囊尾蚴并记录其在兔体内的寄生部位.用豆状囊尾蚴经口感染实验犬,观察犬首次排出孕节的时间、每天排出孕节的数目及排孕节持续时间;选取55个孕节,记录孕节中虫卵的数目及大小;用1%次氯酸钠溶液激活虫卵,观察虫卵孵化过程. 结果 5只犬在感染豆状囊尾蚴后分别在感染后39 d、43 d、45 d、50 d和57 d开始排出孕节;最多一天排出158片孕节;孕节中虫卵含量在60~26 480个之间,平均为3372个,虫卵大小为(41.32±4.53) μm×(36.23±4.92) μm;虫卵孵化需要4~5 min.豆状囊尾蚴在家兔体内主要寄生于胃大网膜和直肠浆膜. 结论 犬感染豆状囊尾蚴后,在犬体内发育成熟的豆状带绦虫排孕节持续时间长,孕节内虫卵量大,排出的虫卵可对环境造成较大的污染,极易引起中间宿主的感染.
英文摘要： Objective To further investigate the biological characteristics of Taenia pisiformis Sichuan strain, as well as to provide basic knowledge for future prevention and control of Cysticercus pisiformis infection. Methods Eight rabbits were infected with T. pisiformis eggs and then killed for larvae collection on day 60 post-infection (PI). Meanwhile, the number and location of C. pisiformis were determined. Subsequently, five dogs were infected with C. pisiformis under standard experimental conditions. The time of first discharged gravid proglottids, the number of daily evacuated gravid prolottids and the duration of gravid proglottids evacuation were recorded. Furthermore, fifty-five gravid proglottids were selected to examine the numbers and sizes of eggs in each gravid proglottid using microscope. Microscopy was also conducted to observe the hatching process of eggs activated by 1% sodium hypochlorite solution. Methods The infected dogs were first observed evacuating gravid progottids on day 39 PI, 43 PI, 45 PI, 50 PI and 57 PI, respectively. The largest number of gravid proglottids evacuated per day was 158. Representatively, the amount of eggs within a single gravid proglottid ranged from 60 to 26 480, with an average of 3372, while the egg size was (41.32 ± 4.53) μm×(36.23 ± 4.92) μm. Egg hatching took about 4 to 5 min in vitro. In rabbits, C. pisiformis mainly parasitized great omentum and mesentery. Conclusion The discharge of gravid proglottid by mature T. pisiformis lasted for a considerable time, and the gravid proglottid releasing a significant number of eggs, which could cause great environmental contamination and increase the risk of infection in intermediate host. 2015,34(3): 439-443 收稿日期：2014-8-30 DOI：10.3969/j.issn.1000-7083.2015.03.023 分类号：S858.291 基金项目：教育部长江学者和创新团队发展计划(IRT0848); 四川省科技计划项目(2015JY0018) 作者简介：陈林(1982—),男,博士,助理研究员,从事动物寄生虫病学研究,E-mail：chenlin11222@163.com *通讯作者：杨光友,教授,从事动物寄生虫病学研究,E-mail：guangyou1963@hotmail.com 参考文献： 马云祥, 苏云普, 颜秋叶, 等. 2002. 猪带绦虫孕节片内虫卵的数量与成熟率的观察[J]. 中国寄生虫学与寄生虫病杂志, 20(2): 98-100. 牟静, 杨应东, 杨光友, 等. 2010. 多头带绦虫四川株的生物学特性[J]. 中国兽医科学, 40(9): 895-899. 孙晓林, 陈怀涛, 才学鹏, 等. 2008.
