不同滴水量处理下大豆根系生长与花荚形成的关系

[目的] 本文旨在探究逆境下BcGME基因与抗坏血酸(AsA)含量的关系,为培育高品质的不结球白菜品种奠定理论基础。[方法] 以不结球白菜’苏州青’为材料,同源克隆了BcGME基因的全长,应用生物信息学分析了其氨基酸序列,通过实时荧光定量PCR技术测定了BcGME基因在过氧化氢胁迫和水淹胁迫下的表达水平,利用高效液相色谱(HPLC)测定了相应的抗坏血酸含量。[结果] BcGME基因包含一个长度为1 137 bp的开放式阅读框(ORF),编码379个氨基酸。推测其蛋白理论相对分子质量为42.97×103,理论等电点pI值为5.84,分子式为C1907H2949N519O570S22,属于不稳定蛋白。总平均疏水指数为-0.440,属于亲水蛋白。亚细胞定位预测该蛋白分布在细胞质中。BcGME氨基酸序列与十字花科大部分植物的相似性为90%以上,不结球白菜BcGME与十字花科的大白菜、甘蓝型油菜、拟南芥相应蛋白的进化距离较近。过氧化氢胁迫下,AsA含量在6 h达到峰值,同时BcGME基因的表达量也达到最高;水淹胁迫下,1 d时AsA含量最高为12.99 μg？g-1,随后AsA含量急速下降,1 d时的BcGME基因表达量是0 d的1.5倍。[结论] 过氧化氢胁迫和水淹胁迫下,不结球白菜植株中BcGME基因的表达量变化趋势与AsA含量变化趋势一致,说明GME可能是不结球白菜抗坏血酸合成途径中的关键调控基因。
[Objectives] The project aims to provide the relationship of root growth and flower-pod formation of the high yield soybean. [Methods] In the field,there were four different drop irrigation amount treatments i.e.W1(900 m3？hm-2),W2(1 725 m3？hm-2),W3(2 550 m3？hm-2)and W4(3 375 m3？hm-2),and author studied systematically the effect of soil moisture content in 0-120 cm and proved the relationship among the total dry root weight(ditch method),total lateral root length(cross-grid method)and flower-pod formation. [Results] The results showed that the effects were significant by increasing drop irrigation amount on the upper and lower limit of moisture content in 0-40 cm soil layers,but it was indistinctive in 40-60 cm soil layers,and the moisture content presented a downward trend. Increasing drop irrigation amount mainly promoted the growth of dry root weight density and lateral root length in 0-40 cm soil layers,and led to improve the total dry root weight and total lateral root length,and the effect were not obvious on dry root weight and root length in 40-80 cm layers. Increasing the amount of drip irrigation improved obviously the total number of flowers,which mainly enhanced the number of flowers of the 10 to 15 node,prolonged the florescence,postponed the date of beginning pod appearance,prolonged the bearing pod phase,and made the nodes bearing fertile pods higher. Increment of the dry root weight and lateral root length,and relationship of the bleeding potential and pod numbers could be described very well by linear equation in 0-80 cm soil layers at flowering-poding phase,and their correlation coefficients were 0.900 1,0.804 5 and 0.970 8(P<0.05). [Conclusions] Drop irrigation amount at flowering-poding phase was raised,the root volume growth was promoted very well,the amount of flowers and pods was increased,and then the yield of soybean was raised