罗望子多糖分级提取及其与黄原胶凝胶骨架材料缓释性能研究

将罗望子多糖胶粉分别在50和100℃(TSP50和TSP100)条件下分级提取与纯化,其提取得率分别为28.71%和36.24%。X射线衍射图谱结果表明,在100℃下提取的罗望子多糖分级组分的结晶度明显高于50℃条件下提取的组分的结晶度。采用湿法造粒制备罗望子分级多糖与黄原胶不同质量配比的控释茶碱的缓释材料,并比较分析了其缓释性能。体外释药结果表明,在药物释放12 h时,仅含罗望子多糖骨架片的药物累积释放率最大,TSP50和TSP100分别达到载药量的93.4%和81.6%。当罗望子多糖和黄原胶的质量比例为40:60时,TSP50和TSP100的药物累积释放量最小,分别为67.2%和67.4%,表现出最为持续的药物释放性能。溶胀性能结果表明,罗望子多糖和黄原胶质量比例为40:60时,制备的骨架片均在溶胀6 h时表现出最高溶胀率,TSP50和TSP100溶胀率分别为285%和312%。
Tamarind seed polysaccharide(TSP)was fractional extracted from tamarind seed powder at 50℃ and 100℃, and the TSP yields were 28.71% and 36.24%, respectively. The main monosaccharide components in both two fraction TSPs(TSP50 and TSP100)were xylose, galactose and glucose with a ratio of 1.00:1.48:2.85 and 1.00:1.65:3.20, respectively. The FT-IR spectra of the TSP displayed peaks at 1 376 cm-1, 1 039 cm-1, 943 cm-1 and 899 cm-1, which were the characteristic absorption peaks of tamarind seed polysaccharide. Results from X-ray diffractograms(XRD)showed that the TSP samples extracted at 50℃ and 100℃ both presented a broad band centered at 2θ=19.4°. The crystallinity of the TSP100 was much higher than that of TSP50. The matrix tablets of theophylline were prepared by a wet granulation technique by using TSP and xanthan as raw materials. The ratios of tamarind seed polysaccharide(TSP50 and TSP100):xanthan in the matrix tablets were 20:80, 40:60, 60:40, 80:20, 100:0(TX20:80 to TX100:0). The in vitro drug release test showed that the cumulative drug release rate from the matrix tablets containing only TSP50 or TSP100 reached 93.4% and 81.6% of original drug within 12 h, respectively. While the most sustained drug release was found in the formulation of TX40:60 with both TSP50 and TSP100 matrices, which released 67.2% and 67.4% of original drug within 12 h, respectively. These results could be attributed to the viscosity and swelling properties of two polysaccharides. The results of swelling properties showed that the maximum swelling rate were 285% and 312% of the matrix tablets of TX40:60 and xanthan gum. We also found that the swelling index of the TSP100 was obviously higher than that of the TSP50, which might be due to a more difficult dissolution of the TSP100 with a higher molecular weight