超高压体系下皱褶假丝酵母脂肪酶的水解特性

研究了压力、温度、pH、保压时间对脂肪酶活性的影响，并分析了酶的复原性，测定了不同温度和压力处理脂肪酶的动力学参数。结果表明，200 MPa时脂肪酶酶活最高，是常压的1.76倍。200 MPa时脂肪酶最适温度为40 ℃，最适pH值7.5，最佳保压时间为10 min。与常压相比，200 MPa时，40 ℃时Km值变化不大，50 ℃时Km值有所增加，而Vmax、Kcat、Kcat/Km值随压力的升高而增大。
The effects of pressure，temperature，pH and holding time on Candida rugosalipase activity were studied and the recovery of the enzyme was analyzed. We also investigated the changes of kinetics parameters of lipase under different temperature and pressure. The results showed that lipase activity was the highest under 200 MPa and almost 1.76 times of atmospheric pressure. Under this pressure，the optimum temperature and pH for lipase were 40 ℃and pH 7.5，respectively；the processing time was 10 min；the change of Km at 40 ℃ is not as obvious as 50 ℃；meanwhile the Vmax，Kcat and Kcat/Km increased significantly with the elevated pressure