豚鼠耳蜗基底膜振动的测试方法

Objective To explore a method to measure the vibration in basilar membrane of guinea pig′s cochlea.Methods Guinea pig was under pentobarbital anesthesia by intraperitoneal injection throughout the experiments.We made a ventrolateral incision and dissected the muscles.Then the bulla could be seen at the deep side of the mandible.The bulla was exposed by scraping the muscles on its surface,and was opened by electric drill.After the location of the basilar membrane was distinguished,a circle ditch was drilled gradually on corresponding bony wall.Then a fine hole of the bony wall could be done by openng this small piece isolated bony wall with a pointed scalpel.The reflected glass beads were placed on the basilar membrane using a pointed stainless needle.Then the laser doppler vibrometer (LDV) was used to record the vibration in basilar membrane under pure tone stimulation from the outer ear.Results We measured the response curve of basilar membranem in guinea pig′s cochlea under sound stimulation of different frequencies. Conclusions This method can be used to measure the vibration in basilar membrane of guinea pig′s cochlea in vitro,while in vivo test may need further research.