连花急支片在慢性支气管炎大鼠中的药效学研究

Objective To study the effectiveness of Lianhua Jizhi Tablets for chronic bronchitis.Methods Seventy-two SD rats were assigned to six groups:control group,smoking group,smoking and high-dose Lianhua group,smoking and medium-dose Lianhua group,smoking and low-dose Lianhua group and smoking and acetylcysteine group.The rat model of chronic bronchitis was established by daily exposure to cigarette smoking and then treated with different treatments.Airway responsiveness was assessed by pulmonary function meter.HE staining of lung tissue was used to analyze the degree of inflammatory response and the pathological changes.ELISA was used to detect the concentration of inflammatory cytokines in serum and bronchoalveolar lavage fluid (BALF).Results After Lianhua Jizhi Tablets and acetylcysteine treatment,the performance status of smoking rat was significantly elevated.Lianhua Jizhi Tablets and acetylcysteine treatment could significantly improve the airway responsiveness of smoking rats.HE staining showed that infiltration of inflammatory cells in bronchia and edema of bronchial wall were alleviated by Lianhua Jizhi Tablets and acetylcysteine treatment.Lianhua Jizhi Tablets and acetylcysteine treatment could significantly reduce the levels of IL-4,IL-13 and IFN- γ in BALF of smoking rats.Levels of IL-6 and TNF-α in serum decreased at different degrees by Lianhua Jizhi Tablets and acetylcysteine treatment.Conclusions Similar to acetylcysteine,Lianhua Jizhi Tablets alleviated the infiltration of inflammatory cells,pathological changes and improved pulmonary function in rats through regulating the balance of inflammatory cytokines,and consequently achieved a therapeutic effect.