Background: To explore the hepatoprotective effect of Yigan mingmu oral liquid
(YGMM) on isoniazid-rifampicin induced liver injury in rats. Methods: Total
38 SD rats were randomly divided into 6 groups including
control group, model group, silymarin positive control group, and three YGMM
treatment groups. Model group was administered intragastrically with INH (100
mg/kg) and RIF (100 mg/kg) for 14 days. Silymarin group and YGMM treatment groups were administered intragastrically with silymarin (100
mg/kg) and different doses of YGMM (1, 2.5, 5 mg/kg) 2 hours before INH and RIF
administration from day 4 to day 14.？Results: Rats
were sacrificed 16 hours after the last day treatment to determine the
activities of serum alanine transaminase (ALT), aspartate transaminase (AST)
and alkaline phosphatase (ALP), as well as total bilirubin (TB) content. Oxidative stress was evaluated by measuring total superoxide
dismutase (T-SOD) and malondialdehyde (MDA) levels. Histopathological changes
in liver tissues were observed under an optical microscope by using hematoxylin
and eosin staining. The mice？in model groups showed
significantly (p < 0.05) increased levels in AST, ALT, ALP, TB and
MDA compared to their control groups; and showed significantly (p <
0.05) decreased level in T-SOD. These changes were significantly (p <
0.05) reversed by the YGMM treatments in a
dose-dependent manner. Hepatic pathological changes were attenuated or even
reversed by silymarin or YGMM treatments. Conclusions: YGMM has a good hepatoprotective activity on isoniazid-rifampicin induced liver
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