A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-washing, elution, pressure filtration, dialysis, spray drying. Activity of the product was measured using a heparin anti-IIa factor assay kit. The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method; the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine. The activity was 98.48 ± 2.49 IU/mg (n = 5), 15.18 IU/mg higher than that obtained by oven drying method. The product is pale white powder, attractive color and easy to dissolve.
References
[1]
Faham, S., Hileman, R.E., Fromm, J.R., Linhardt, R.J. and Rees, D.C. (1996) Heparin Structure and Interactions with Basic Fibroblast Growth Factor. Science (New York), 271, 1116-1120. https://doi.org/10.1126/science.271.5252.1116
[2]
Buyue, Y., Misenemer, T.M. and Sheehan, J.P. (2012) Low Molecular Weight Heparin Inhibits Plasma Thrombin Generation via Direct Targeting of Factor IXa: Contribution of the Serpin-Independent Mechanism. Journal of Thrombosis and Haemostasis, 10, 2086-2098. https://doi.org/10.1111/j.1538-7836.2012.04892.x
[3]
Mauri, L., Boccardi, G., Torri, G., Karfunkle, M., Macchi, E., Muzi, L., Keire, D. and Guerrini, M. (2017) Qualification of HSQC Methods for Quantitative Composition of Heparin and Low Molecular Weight Heparins. Journal of Pharmaceutical and Biomedical Analysis, 136, 92-105.
[4]
Li, S.W., Feng, X., Xu, H. and Chen, K.J. (2018) Comparison on Anticoagulation and Antiplatelet Aggregation Effects of Puerarin with Heparin Sodium and Tirofiban Hydrochloride: An In Vitro Study. Chinese Journal of Integrative Medicine, 24, 103-108. https://doi.org/10.1007/s11655-017-2419-7
[5]
Ma, L.Y., Liu, H. and Jiang, Z.W. (2012) Research Progress of Anti-Tumor Drugs Related to Heparin and Heparan Sulfate. Chinese Pharmacology Bulletin, 28, 1193-1196.
[6]
Caruana, R.J., Smith, M.C. and Clyne, D. (1991) Controlled Study of Heparin versus Epoprostenol Sodium (Prostacyclin) as the Sole Anticoagulant for Chronic Hemodialysis. Blood Purification, 9, 296-304. https://doi.org/10.1159/000170027
[7]
Wu, D.M. (2010) Anticoagulation Therapy for Acute Deep Vein Thrombosis: Selection and Application of Heparin. Chinese Journal of Practical Surgery, 30, 1033-1034.
[8]
Dong, H., Ding, L.X. and Luo, Z.L. (2014) Study on the Technology of High Efficiency Extraction of Heparin Sodium by Enzymatic Method Combined with Membrane Separation. Journal of Food Industry, 35, 158-160.
[9]
Chen, Z. (2011) Improvement of Heparin Post-Treatment Process and Study of Low Molecular Weight Heparin Production Process. Huazhong University of Science and Technology, Wuhan.
[10]
Mironoy, A.N., Goryachev, D.V. and Garvishina, E.V. (2015) Specific Anti-IIa Activity is a Key Indicator of Safety and Efficacy in Validation of Biosimilarity of Unfractioned Heparin Preparations. Bulletin of Experimental Biology and Medicine, 158, 450-452. https://doi.org/10.1007/s10517-015-2782-3
