Background:Alpinia
officinarumHance is valued as an edible
medicinal plant. The rhizome is widely reported to have anticancer activity
whereas little information is available on the aerial part. This study
investigates chemical composition, proapoptotic and anti-osteoporosis
activities of essential oil from aerial parts of A. officinarum (APEO). Methods: In this study, APEO was extracted by hydrodistillation and
analyzed using GC-MS. The inhibitive
activity of 0-2.5 μL/mL. APEO was investigated using
MTT assay, while in vivo effect was evaluated in nude
mice. The cell cycle, apoptosis, Δψm
and expression of proteins analyses influenced by 0-0.313 μL/mL
APEO were detected by PI, Annexin V/PI, JC-1, and Western blot, respectively. Alkaline
phosphatase activity and mineralized nodules formation of rat osteoblasts with
0-0.156 μL/mL APEO were assayed using colorimetric method
and alizarin red staining, respectively. Results: Total 45 constituents were identified accounting for 91.1% of
APEO (sesquiterpene hydrocarbons for 44.4%). APEO significantly inhibited cancer
cells growth in a dose-dependent
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