Provisional prosthetic restoration materials are exposed to oral cavity producing on the surface biofilm where different factors such as surface roughness and porosity can condition their formation and organization and can create stagnation areas that promote the bonding of organic particles, thus facilitating the formation and maturation of the biofilm. The purpose of this study was to compare surface roughness of two provisional prosthetic restorations materials and their bacterial susceptibility. In this study, two provisional restoration materials were used in two groups, A polymethyl methacrylate acrylic (NicTone MDC DENTAL) and B bis-acryl resin (Protemp 4 ESPE 3M). A total of 80 samples (40 samples of each material) were in thick plates of 10 × 10 mm and 2 mm high. 20 samples of each material were polished, while 20 were left unpolished. Subsequently, the samples were observed by Atomic Force Microscopy for their evaluation of surface roughness. The values were analyzed with t-test, Mann-Whitney U test and Kruskal Wallis test. The samples were microbiologically inoculated with the strains obtained and identified from a provisional polymethyl methacrylate acrylic restoration in a patient, in order to observe bacterial adhesion using a Scanning Electron Microscope. Two strains, Enterococcus faecalis and Pseudomona luteola, were identified. The presence of the microorganisms was observed on the surface of both materials, either polished or unpolished, with a lower level of microorganism adhesion found on the bis-acrylic resin. There was a significant difference about surface roughness in the groups A and B with p < 0.05; meanwhile there was also a significant difference between polished polymethyl methacrylate and polished bis-acrylic resin (p = 6.7 × 10-8). We found that the polished bis-acrylic resin showed lower surface roughness and bacterial adhesion in comparison with the polymethyl methacrylate.
References
[1]
Burns, D.R, Beck, D.A. and Nelson, S.K. (2003) A Review of Selected Dental Literature on Contemporary Provisional Fixed Prosthodontic Treatment: Report of the Committee on Research in Fixed Prosthodontics of the Academy of Fixed Prosthodontics. The Journal of Prosthetic Dentistry, 90, 474-497.
https://doi.org/10.1016/S0022-3913(03)00259-2
[2]
Ayuso-Montero, R., Martinez-Gomis, J., Lujan-Climent, M., Salsench, J. and Peraire, M. (2009) Influence of Matrix Type on Surface Roughness of Three Resins for Provisional Crowns and Fixed Partial Dentures. Journal of Prosthodontics, 18, 141-144. https://doi.org/10.1111/j.1532-849X.2008.00392.x
[3]
Guler, A.U., Kurt, S. and Kulunk T. (2005) Effects of Various Finishing Procedures on the Staining of Provisional Restorative Materials. The Journal of Prosthetic Dentistry, 93, 453-458. https://doi.org/10.1016/j.prosdent.2005.02.001
[4]
Sen, D., Göller, G. and Issever, H. (2002) The Effect of Two Polishing Pastes on the Surface Roughness of Bis-Acryl Composite and Methacrylate-Based Resins. The Journal of Prosthetic Dentistry, 88, 527-532.
https://doi.org/10.1067/mpr.2002.129335
[5]
Buergers, R., Rosentritt, M. and Handel, G. (2007) Bacterial Adhesion of Streptococcus mutans to Provisional Fixed Prosthodontics Material. The Journal of Prosthetic Dentistry, 98, 461-469. https://doi.org/10.1016/S0022-3913(07)60146-2
[6]
Kuhar, M. and Funduk, N. (2005) Effects of Polishing Techniques on the Surface Roughness of Acrylic Denture Base Resins. The Journal of Prosthetic Dentistry, 93, 76-85. https://doi.org/10.1016/j.prosdent.2004.10.002
[7]
Rüttermann, S., Bergmann, N., Beikler, T., Raab, W.H. and Janda, R. (2012) Bacterial Viability on Surface-Modified Resin-Based Dental Restorative Materials. Archives of Oral Biology, 57, 1512-1521.
https://doi.org/10.1016/j.archoralbio.2012.05.005
[8]
Bollen, C.M., Lambrechts, P. and Quirynen, M. (1997) Comparison of Surface Roughness of Oral Hard Materials to the Threshold Surface Roughness for Bacterial Plaque Retention: A Review of the Literature. Dental Materials, 13, 258-269.
https://doi.org/10.1016/S0109-5641(97)80038-3
[9]
Morgan, T.D. and Wilson, M. (2001) The Effects of Surface Roughness and Type of Denture Acrylic on Biofilm Formation by Streptococcus oralis in Constant Depth Film Fermentor. Journal Applied Microbiology, 91, 47-53.
https://doi.org/10.1046/j.1365-2672.2001.01338.x
[10]
Sardin, S., Morrier, J.J., Benay, G. and Barsotti, O. (2004) In Vitro Streptococcal Adherence on Prosthetic and Implant Materials. Interactions with Physicochemical Surface Properties. Journal of Oral Rehabilitation, 31, 140-148.
https://doi.org/10.1046/j.0305-182X.2003.01136.x
[11]
Gharechahi, M., Moosavi, H. and Forghani, M. (2012) Effect of Surface Roughness and Materials Composition on Biofilm Formation. Journal of Biomaterials and Nanobiotechnology, 3, 541-546. https://doi.org/10.4236/jbnb.2012.324056
[12]
Busscher, H.J., Rinastiti, M., Siswomihardjo, W. and van der Mei, H.C. (2010) Biofilm Formation on Dental Restorative and Implant Materials. Journal of Dental Research, 89, 657-665. https://doi.org/10.1177/0022034510368644
[13]
Quirynen, M. and Bollen, C.M. (1995) The Influence of Surface Roughness and Surface-Free Energy on Supra- and Subgingival Plaque Formation in Man. A Review of the Literature. Journal of Clinical Periodontology, 22, 1-14.
https://doi.org/10.1111/j.1600-051X.1995.tb01765.x
[14]
Quirynen, M., Marechal, M., Busscher, H.J., Weerkamp, A.H., Darius, P.L. and van Steenberghe, D. (1990) The Influence of Surface Free Energy and Surface Roughness on Early Plaque Formation. An in Vivo Study in Man. Journal of Clinical Periodontology, 17, 138-144. https://doi.org/10.1111/j.1600-051X.1990.tb01077.x
[15]
Liljemark, W.F. and Bloomquist, C. (1996) Human Oral Microbial Ecology and Dental Caries and Periodontal Diseases. Critical Reviews in Oral Biology and Medicine, 7, 180-198. https://doi.org/10.1177/10454411960070020601
[16]
Montanaro, L., Campoccia, D., Rizzi, S., Donati, M.E., Breschi, L., Prati, C. and Arciola, C.R. (2004) Evaluation of Bacterial Adhesion of Streptococcus mutans on Dental Restaurative Materials. Biomaterials, 25, 4457-4463.
https://doi.org/10.1016/j.biomaterials.2003.11.031
[17]
Sundqvist, G., Figdor, D., Persson, S. and Sjögren, U. (1998) Microbiologic Analysis of Teeth with Failed Endodontic Treatment and the Outcome of Conservative Re-Treatment. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 85, 86-93. https://doi.org/10.1016/S1079-2104(98)90404-8