The objective of this study is the characterization of mould inside and in the surface wall of a hospital. The present research was made on the wall of the Clinical Hospital of the Federal University of Paraná, Brazil. For the methodology the samples were extracted from the surface, mortar and brick. The samples were spread on Petri plates containing Sabouraud dextrose agar and incubated at 25℃ for seven days. The results of the 90 samples collected showed growth of 39% of colonies with the following distribution of microorganisms: Aspergillus (present in 27% of samples), Cladosporium, Absidia, Rhizopus, Rhodotorula, Fusarium, Penicillium and Aspergillus flavus. Within the investigated substrate, three species of different fungi were identified: Aspergillus flavus, Aspergillusfumigatus and Aspergillus niger.
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