Effects of Nourishing “Yin”-Removing “Fire” Chinese Herb Mixture on the Expression of GABAB Receptors in Hypothalamus of Precocious Puberty Female Rats
Objectives: To investigate the potential role of GABAB Receptors (GABABRs)
involved in the effect of Nourishing “Yin”-Removing “Fire” Chinese herb mixture
(TCM) treatment on precocious puberty. Methods: Female Sprague-Dawley rats were
randomly divided into four groups: normal (N), central precocious puberty (CPP)
model (M), CPP fed with normal saline (S) and CPP fed with Nourishing
“Yin”-Removing “Fire” Chinese herb mixture (TCM). Rats of postnatal day 5 were
given a single subcutaneous injection of 240 μg danazol to establish CPP model
rats. Rats of S and TCM groups were continuously administered with saline or
nourishing “Yin”-removing “Fire” Chinese herb mixture since postnatal day 15.
The expression of GABABRs was detected by means of real-time PCR and
immunohistochemistry. Results: The expression of hypothalamic GnRH mRNA in M was
significantly increased on the day of pre-puberty when compared with that of N (P
< 0.01). On the day of onset-puberty, LH levels were higher in M than those
in N (P < 0.01), while the serum E2 and LH levels of TCM decreased when compared
with those of M (P < 0.05). On the day of pre-puberty, the number of GABAB1 receptor (GABABR1) immunoreactive cells in the arcuate nucleus (ARN) was
decreased in M when compared with that of N (P < 0.05) and increased in TCM
compared with that of M (P < 0.05); simultaneously, the expression of
GABABR1 mRNA in hypothalamus was significantly decreased in M when compared
with that of N (P < 0.01) and increased in TCM compared with that of M (P <
0.01). On the day of onset-puberty, the number of GABABR1 immunoreactive cells
in medial septum (MS) was decreased in M compared with that in N (P < 0.05)
and increased in TCM comparing with that of M (P < 0.05); meanwhile, the
mRNA expression of GABABR1 in hypothalamus was decreased in M compared with
that in N (P < 0.05), while the numbers of GABAB2 receptor
(GABABR2) immunoreactive cells in the ARN, MS and diagnonal band of broca (DBB)
were decreased in M when compared with those of N (P < 0.05) and increased
in TCM compared with those of M (P < 0.05) on the day of onset-puberty. At
the same time, the mRNA expression of GABABR2 was significantly decreased in M
compared with that of N (P < 0.01). Conclusions: These results indicated
that the GABABRs might
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