Objective: To
investigate effects of Cornus officinalis Total Glycosides (COTG) and Cornus Polysaccharide (CP) on myocardial protection
and on expression of mitochondria biogenesis related gene of acute myocardial
infarction (AMI) rats, Materials and Methods: Ninety-six SD rats of SPF level were
randomly divided into 5 groups: sham operation group, model group, preventive
treatment group, COTG treatment group, CP treatment group, and there were 12
cases in each one. By legating the left anterior descending branch of coronary
artery method, acute myocardial infarction model was established. The rat of sham
operation group and model group was intragastric administered with physiological
saline; other groups were given with corresponding drugs. The cardiac function,
the myocardial infarct area, the expression of mitochondrial biogenesis genes
such as PGC-1α, PGC-1β, NRF-1mRNA and GSK-3β mRNA, GSK-3β Protein Expression were analyzed. Results: The results revealed
that compared with model group, myocardial infarction size, LVDs, LVDd, LVESV, LVEDP,
and -dp/dt decreased; LVSP increased in preventive
treatment group, COTG treatment group, and CP treatment group (p < 0.05); LVEDV
increased in preventive treatment group (p < 0.05), PGC 1 alpha, and PGC 1
beta; the NRF-1 mRNA expression increased in preventive treatment group, COTG
treatment group, and CP treatment group (p < 0.05). Compared with CP and
COTG treatment group, PGClpha, beta PGC 1, the NRF-1 mRNA expression increased in
preventive treatment group (p < 0.05). Compared with the sham operation
group, GSK-3 beta mRNA and protein expression increased in model group, preventive
treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared
with model group, GSK-3 beta mRNA expression reduced in preventive treatment
group, COTG treatment group, and CP treatment group (p < 0.05). Conclusions:
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