Objective: The objective of this article is to investigate the effect of Guhanyangshengjing
Tablet (GT) on expression of synaptonemal complex protein 3 (SYCP3), a meiotic
marker, in the testis tissue of aging male rats. Methods: Forty aging male rats
were randomly assigned into 4 equal groups (n = 10 per group). Rats in each group
were treated with GT at dose of 0 (control), 1.5 g/kg, 3.0 g/kg or 4.5 g/kg
respectively by gavage daily for 30 days. At the end of the experiment, all animals
were sacrificed and the blood samples were drawn to evaluate serum testosterone
levels. The reproductive organs of each rat were taken and weighted. The right
testis of each rat was removed for the analysis of intratesticular testosterone
(ITT) concentrations, and the left one was used for immunohistochemical
staining. Results: Compared with the control, reproductive
organs’ weights, serum testosterone levels, ITT concentrations, quality of
sperm, and expression of SYCP3 in the GT-treated groups
were all improved in a dose-dependent manner. Conclusion: GT can improve
testosterone synthesis and promote spermatogenesis simultaneously, indicating
that GT is suitable for late-onset hypogonadism (LOH) patients with fertility
requirements.
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