|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
一株生长ph较宽的产甲烷菌分离与系统发育分析
DOI: 10.3724/SP.J.1145.2009.00554, PP. 554-558
Keywords: 产甲烷菌,菌种分离,厌氧发酵,生长ph,16srdna序列,系统发育分析
Abstract:
采用hungate厌氧操作技术，从造纸厂阴沟污泥中分离到一株生长ph范围为5.5~9.5的产甲烷菌株sh4.该菌对酸碱具有良好的适应性，培养3d后，在初始ph值6.0~8.0的培养基中甲烷产量相差不大，且基本达到最大产量.sh4革兰氏染色阳性，短杆状，多数单生，不运动；菌落近圆形，微黄；利用h2+co2或甲酸盐作为唯一碳源生长，不利用乙酸盐，对氯霉素非常敏感.该菌最适生长ph为7.0，最适生长温度为35℃，最适nacl浓度为0~1.5%.实验表明，与仅添加厌氧污泥作为接种物相比，添加sh4菌液可使产甲烷启动时间缩短1/3，甲烷总产量亦有大幅提高.形态、生理生化特征以及16srdna序列分析表明，该菌为嗜树木甲烷短杆菌(methanobrevibacterarboriphilus).图8参16
| [1] | 1sunz(孙征),zhouyg(周宇光),dongxz(东秀珠).characterizationandphylogeneticsofanewspeciesofgenusmethanobacterium.actamicrobiolsin(微生物学报),2001,41(3):265~269
|
| [2] | 2garciajl,patelb,ollivierb.taxonomic,phylogenetic,andecologicaldiversityofmethanogenicarchaea.anaerobe,2000,6:205~226
|
| [3] | 3patelgb,sprottgd,feinje.isolationandcharacterizationofmethanobacteriumespanolaesp.nov.,amesophilic,moderatelyacidiphilicmethanogen.intjsystbacteriol,1990,40:12~18
|
| [4] | 11cuzinn,ouattaraas,labatm,garciajl.methanobacteriumcongolensespnov,fromamethanogenicfermentationofcassavapeel.internjsyst&evolmicrobiol,2001,51(2):489~493
|
| [5] | 12wrightad,pimmc.improvedstrategyforpresumptiveidentifieationofmethanogensusing16sriboprinting.mierobiolmethods,2003,55(2):337~349
|
| [6] | 14duarteac,andersongk.inhibitionmodelinginanaerobicdigestion.watscitechnol,1982,14:749~763
|
| [7] | 15linxf(凌雪峰),zuoje(左剑恶),guxs(顾夏声).studiesoncultivationofacid-tolerantmethanogenicgranlularsludgeusingriversediments.chinaenviron(中国环境科学),2003,23(6):587~591
|
| [8] | 16zuoje(左剑恶),linxf(凌雪峰),guxs(顾夏声).astudyoncultivationofacid-tolerantmethanogenicgranularsludgeunderlowphvaluesanditscharacteristics.microbiology(微生物学通报),2004,31(5):65~69
|
| [9] | 4liuy,boonedr,choyc.methanohalophilusoregonensesp.nov.,amethylotrophicmethanogenfromanalkaline,salineaquifer.intjsystbacteriol,1990,40:111~116.
|
| [10] | 5mathraniim,boonedr,mahra,foxge,laupp.methanohalophiluszhilinaesp.nov.,analkaliphilic,halophilic,methylotrophicmethanogen.intjsystbacteriol,1988,38:139~142
|
| [11] | 6kevbrinvv,lysenkoam,zhilinatn.physiologyofthealkaliphilicmethanogenz-7936,anewstrainofmethanosalsuszhilinaeaeisolatedfromlakemagadi.microbiolengltr,1997,66:261~266
|
| [12] | 7bräuersl,yashiroe,uenong,yavittjb,zindersh.characterizationofacid-toleranth2/co2-utilizingmethanogenicenrichmentculturesfromanacidicpeatboginnewyorkstate.femsmicrobiolecol,2006,57:206~216
|
| [13] | 8钱泽澍,闵航.沼气发酵微生物学.杭州:浙江科学技术出版社,1986.274~275
|
| [14] | 9hungatere.aroll-tubemethodforcultivationofstrictanaerobes.methodsmicrobiol,1969,3b:117~132
|
| [15] | 10gongg(龚革),wangxy(王修垣).featuresofninestrainsofthermophilicmethanogens.actamicrobiolsin(微生物学报),1997,37(5):378~384
|
| [16] | 13农业部厌氧微生物重点开放实验室.产甲烷菌及其研究方法.成都:成都电子科技大学出版社,1997.223~224
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
