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OALib Journal期刊
ISSN: 2333-9721
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沙里院褐球蚧蜜露对病原真菌蜡蚧轮枝菌侵染力的影响
DOI: 10.3724/SP.J.1145.2010.00504, PP. 504-508
Keywords: 沙里院褐球蚧,蜜露,昆虫病原真菌,蜡蚧轮枝菌,侵染力,蛋白酶,几丁质酶
Abstract:
蚧虫在取食和发育过程中排泄大量蜜露，通过研究蜜露对病原真菌侵染蚧虫的影响，可为应用病原真菌防治蚧虫提供科学依据.于2007年4月自山西省太原市苹果树上沙里院褐球蚧rhodococcussariuoniborchsenius雌虫虫体表面采取蜜露，用化学分析方法和氨基酸自动分析仪测定其化学成份；将蜜露稀释为10%、20%、30%、40%、50%共5个浓度，对蜡蚧轮枝菌（verticilliumlecanii）的两个菌株no.v3.4504和no.v3.4505进行培养，比较其对菌株胞外蛋白酶和几丁质酶活性的影响.结果发现，该蚧虫蜜露包含可溶性糖（占69%）、水分（占26%）和18种氨基酸.当蜜露稀释浓度为10%时，菌株no.v3.4504的蛋白酶活性最大，为(4.45±0.18)u/ml；随着蜜露浓度增加，蛋白酶活性呈下降趋势；在蜜露浓度为20%时，菌株no.v3.4505的蛋白酶活性出现最大值，为(8.5±0.71)u/ml；随后，蛋白酶活性持续降低.两菌株几丁质酶的活性与蛋白酶活性有相同的变化趋势.在培养基中分别加入氨基酸ala、met、gly、pro，发现加入met使菌株no.v3.4504和no.v3.4505的蛋白酶活性分别增长了256.9%和215.7%.表明低浓度的蜜露和培养基中加入适当met能提高蜡蚧轮枝菌对蚧虫的侵染力.图3表3参14
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