|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
甘薯蔗糖转运蛋白ibsut1x在酵母细胞中的定位
DOI: 10.3724/SP.J.1145.2010.00798, PP. 798-802
Keywords: 甘薯,蔗糖转运蛋白,基因表达,定位,绿色荧光蛋白,酿酒酵母
Abstract:
蔗糖转运蛋白是植物特有的一类载体蛋白，酿酒酵母突变株susy7/ura3常被利用来研究蔗糖转运蛋白的功能，但在研究中发现，有的蔗糖转运蛋白在酵母中未表现出蔗糖转运的功能.但这或许并不是其实际功能的体现，而是由于这类蛋白无法准确定位到酵母的细胞膜上造成的.ibsut1x是本实验室从甘薯中分离到的一个蔗糖转运蛋白基因，但是在susy7/ura3中未表现出明显的蔗糖转运功能.本研究通过用激光共聚焦显微镜对ibsut1x与绿色荧光蛋白（greenfluorescentprotein，gfp）的融合蛋白的表达和定位的观察，发现ibsut1x不仅在酵母细胞中有表达，而且表达的蛋白质定位在酵母的细胞膜上.结果表明，ibsut1x在酵母细胞中不能转运蔗糖，该蛋白很可能不具有蔗糖转运功能，或者其与蔗糖的亲和力很低.图4表1参21
| [1] | 1lalondes,wipfd,frommerwb.transportmechanismsfororganicformsofcarbonandnitrogenbetweensourceandsink.annurevplantbiol,2004,55:341~372
|
| [2] | 2sauern.molecularphysiologyofhigherplantsucrosetransporters.febslett,2007,581:2309~2317
|
| [3] | 6riesmeierjw,willmitzerl,frommerwb.isolationandcharacterizationofasucrosecarriercdnafromspinachbyfunctionalexpressioninyeast.emboj,1992,11(13):4705~4713
|
| [4] | 13bürklel,hibberdjm,quickwp,kühnc,hirnerb,frommerwb.theh+-sucrosecotransporterntsut1isessentialforsugarexportfromtobaccoleaves.plantphysiol,1998,118:59~68
|
| [5] | 14riesmeierjw,hirnerb,frommerwb.potatosucrosetransporterexpressioninminorveinsindicatesaroleinphloemloading.plantcell,1993,5:1591~1598
|
| [6] | 15shakyar,sturma.characterizationofsource-andsink-specificsucrose/h+symportersfromcarrot.plantphysiol,1998,118:1473~1480
|
| [7] | 16schmittb,stadlerr,sauern.immunolocalizationofsolanaceoussut1proteinsincompanioncellsandxylemparenchyma:newperspectivesforphloemloadingandtransport.plantphysiol,2008,148:187~199
|
| [8] | 17stadlerr,brandnerj,schulza,gahrtzm,sauern.phloemloadingbythepmsuc2sucrosecarrierfromplantagomajoroccursintocompanioncells.plantcell,1995,7:1545~1554
|
| [9] | 18stadlerr,sauern.thearabidopsisthalianaatsuc2geneisspecificallyexpressedincompanioncells.botacta,1996,109:299~306
|
| [10] | 19truernite,sauern.thepromoterofthearabidopsisthalianasuc2sucrose-h+symportergenedirectsexpressionofp-glucuronidasetothephloem:evidenceforphloemloadingandunloadingbysuc2.planta,1995,196:564~570
|
| [11] | 20schulzew,weisea,frommerwb,wardjm.functionofthecytosolicn-terminusofsucrosetransporteratsut2insubstrateaffinity.febslett,2000,485:189~194
|
| [12] | 3lemoiner.sucrosetransportersinplants:updateonfunctionandstructure.biochimbiophysacta,2000,1465:246~262
|
| [13] | 4kühnc,hajirezaeim-r,ferniear,roessner-tunaliu,czechowskit,hirnerb,frommerwb.thesucrosetransporterstsut1localizestosieveelementsinpotatotuberphloemandinfluencestuberphysiologyanddevelopment.plantphysiol,2003,131:102~113
|
| [14] | 5zhouy,quh,dibleyke,offlerce,patrickjw.asuiteofsucrosetransportersexpressedincoatsofdevelopinglegumeseedsincludesnovelph-independentfacilitators.plantj,2007,49:750~764
|
| [15] | 7lemoiner,bürklel,barkerl,sakrs,kühnc,regnacqm,gaillardc,delrots,frommerwb.identificationofapollen-specificsucrosetransporter-likeproteinntsut3fromtobacco.febslett,1999,454:325~330
|
| [16] | 8liy,guy,qinh,zhangy.twopairsofsucrosetransportersinipomoeabatatas(l.)lamarepredominantlyexpressedinsinkleavesandsourceleavesrespectively,plantsci,2010,179:250~256
|
| [17] | 9newsteads,kimh,vonheijneg,iwatas,drewd.high-throughputfluorescent-basedoptimizationofeukaryoticmembraneproteinoverexpressionandpurificationinsaccharomycescerevisiae.procnatlacadsciusa,2007,104(35):13936~13941
|
| [18] | 10drewd,newsteads,sonoday,kimh,vonheijneg,iwatags.gfp-basedoptimizationschemefortheoverexpressionandpuri?cationofeukaryoticmembraneproteinsinsaccharomycescerevisiae.natprotoc,2008,3(5):784~798
|
| [19] | 11gietzrd,schiestlrh.high-ef?ciencyyeasttransformationusingtheliac/sscarrierdna/pegmethod.natprotoc,2007,2(1):31~34
|
| [20] | 12ausubelfm(奥斯伯),kingstonre(金斯顿),scheidemannjg(赛德曼).shortprotocolsinmolecularbiology.4thed.beijing,china:sciencepress(北京:科学出版社),2005
|
| [21] | 21lujm-y,bushdr.his-65intheproton-sucrosesymporterisanessentialaminoacidwhosemodificationwithsite-directedmutagenesisincreasestransportactivity.procnatlacadsciusa,1998,95:9025~9030
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
