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OALib Journal期刊
ISSN: 2333-9721
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一株中度嗜盐淀粉酶产生菌的分离鉴定及酶活测定
DOI: 10.3724/SP.J.1145.2011.00883, PP. 883-886
Keywords: 嗜盐细菌,菌株鉴定,gracilibacillussp.,系统进化,淀粉酶,酶活测定,高盐碱环境
Abstract:
为开发极端环境工业用酶，从新疆盐碱土微生物中分离得到一株中度嗜盐高产淀粉酶活性菌株h3，其能耐受30%盐浓度和ph11的极端环境.通过形态特征、生理生化实验及16srrna基因序列分析，确定h3属于gracilibacillus属.该菌株能在盐浓度为0~30%的培养基上生长，最适生长盐浓度为5%~10%，最适ph值为8.5.在最适生长盐浓度、ph条件下，其淀粉酶活性可达到4830个活力单位，可用于高盐高碱环境下淀粉的水解.图4表2参18
| [1] | 1orena.industrialandenvironmentalapplicationsofhalophilicmicroorganisms.environtechnol,2010,31(8):825~834
|
| [2] | 3sánchez-porroc,martíns,melladoe,ventosaa.diversityofmoderatelyhalophilicbacteriaproducingextracellularhydrolyticenzymes.japplmicrobiol,2003,94(2):295~300
|
| [3] | 4tamurak,dudleyj,neim,kumars.mega4:molecularevolutionarygeneticsanalysis(mega)softwareversion4.0.molbiolevol,2007,24(8):1596~1599
|
| [4] | 5dongxz(东秀珠),caimy(蔡妙英).handbookofcommonbacteriasystematicidentify.beijing,china:sciencepress(北京:科学出版社),2001
|
| [5] | 6prakashb,vidyasagarm,madhukumarms,muralikrishnabg,sreeramuluk.production,puri?cation,andcharacterizationoftwoextremelyhalotolerantthermostable,andalkali-stableα-amylasesfromchromohalobactersp.tvsp101.procbiochem,2009,44(2):210~215
|
| [6] | 11onishih,sonadak.puri?cationandsomepropertiesofanextracellularamylasefrommoderatehalophilic,micrococcushalobius.applenvironmicrobiol,1979,38(4):616~620
|
| [7] | 12goodwa,hartmanpa.propertiesofamylasefromhalobacteriumhalobium.jbacteriol,1970,104(1):601~603
|
| [8] | 13patels,jainn,madamward.productionofα-amylasefromhalobacteriumhalobium.worldjmicrobiol&biotechnol,1993,9(1):25~28
|
| [9] | 14kobayashit,kanaih,hayashit,akibat,akaboshir,horikoshik.haloalkaliphilicmaltotriose-formingα-amylasefromthearchaebacteriumnatronococcussp.strainah-36.jbacteriol,1992,174(11):3439~3444
|
| [10] | 15coronadomj,vargasc,hofemeisterj,ventosaa,nietojj.productionandbiochemicalcharacterizationofanα-amylasefromthemoderatehalophilehalomonasmeridiana.femsmicrobiollett,2000,183(1):67~71
|
| [11] | 16deutchce.characterizationofasalttolerantextracellulara-amylasefrombacillusdipsosauri.lettapplmicrobiol,2002,35(1):78~84
|
| [12] | 9yandri,suhartatit,hadis.purificationandcharacterizationofextracellularα-amilaseenzymefromlocalebacteriaisolatebacillussubtilisitbccb148.eurjscires,2010,39(1):64~74
|
| [13] | 10hutcheongw,vasishtn,bolhuisa.characterizationofahighlystableα-amylasefromthehalophilicarchaeonhaloarculahispanica.extremophiles,2005,9(6):487~495
|
| [14] | 17machiusm,wiegandg,huberr.crystalstructureofcalciumdepletedbacilluslicheniformisα-amylaseat2.2aresolution.jmolbiol,1995,246(4):545~559
|
| [15] | 18setatime.diversityandindustrialpotentialofhydrolaseproducinghalophilic/halotoleranteubacteria.afrjbiotechnol,2010,9(11):1555~1560
|
| [16] | 2margesinr,schinnerf.potentialofhalotolerantandhalophilicmicroorganismsforbiotechnology.extremophiles,2001,5(2):73~83
|
| [17] | 7liuhh(刘贺红),wanjq(万金泉),mayw(马邕文),wangy(王艳).studyondeterminationofamylaseactivityinactivatedsludgewithdnsspectrophotometry.janhuiagricsci(安徽农业科学),2008,36(33):14369~14371
|
| [18] | 8zhangjq(张继千),wub(吴波),zhengbx(郑冰心),shaoyy(邵云云),zhuqz(朱启忠).enzymaticcharacteristicsofmesophilicamylasefromamarinebacteriumstrain11.genomics&applbiol(基因组学与应用生物学),2010,29(1):71~74
|
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