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OALib Journal期刊
ISSN: 2333-9721
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油菜菌核病菌（sclerotiniasclerotiorum）与细菌间的基因水平转移
DOI: 10.3724/SP.J.1145.2012.00042, PP. 42-48
Keywords: 基因水平转移,油菜菌核病,系统发生树,碱基组成
Abstract:
油菜菌核病（sclerotiniasclerotiorum）是油菜生产上最重要的病害之一，其致病性可能来源于基因水平转移（horizontalgenetransfer，hgt）.为认识其致病原理和寻找新的真菌抑制剂的靶点，首先通过blastp发现其基因xm_001585458.1编码蛋白xp_001585508.1与细菌比对结果中出现低e值3.23e-109和高score值436，暗示存在hgt现象；进一步通过系统进化树的建立，发现该蛋白在进化分枝上更接近于细菌中由streptomycessp.c的nz_cm000832.1基因编码的蛋白zp_07291173；同时核苷酸组成分析也发现该基因与油菜菌核病菌基因组的碱基组成有较大差别，gc含量提高了14.95%.这些结果证明了xm_001585458.1的确存在基因水平转移事件.结构分析和cog蛋白功能分类显示该hgt序列编码蛋白xp_001585508.1具有fa58c_3（coagulationfactors5/8typecdomain）、kelchrepeattype1、galactose-bindingdomain-like、galactoseoxidase/kelch,beta-propeller等保守结构域，应为一个膜蛋白并参与多糖代谢，推测该水平转移基因与s.sclerotiorum在侵染植物时进行细胞壁水解和致病性有关.图3表3参17
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