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OALib Journal期刊
ISSN: 2333-9721
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铁皮石斛多酚和黄酮含量及与抗氧化活性的相关性
DOI: 10.3724/SP.J.1145.2014.04028, PP. 438-442
Keywords: 铁皮石斛,多酚,黄酮,抗氧化
Abstract:
通过测试abts、dpph和羟自由基的清除活性和还原能力，研究铁皮石斛不同极性提取物的体外抗氧化活性及其与多酚和黄酮含量的相关性.采用溶剂提取法制备4个不同极性铁皮石斛提取物（ee、ce、eae、nbe），以vc为阳性对照.结果显示，样品ce中总酚含量为40.96mg/g，黄酮含量为104.48mg/g，在各样品中含量最高.体外抗氧化实验显示，样品ce浓度为375μg/ml时对abts自由基的清除率达到100%，其ic50为88.82μg/ml；ce浓度为1500μg/ml时对dpph自由基的清除率达到78.45%，ic50为394.62μg/ml；ce浓度为1500μg/ml时还原能力为62.29%，随浓度增大到3000μg/ml时还原能力达100%，其ic50为905.06μg/ml.相关性研究显示各提取物对abts、dpph清除能力以及还原性与黄酮含量相关性较强，相关系数分别为0.845、0.902和0.994.而各提取物的还原能力及对羟自由基的清除能力与样品中的多酚含量明显相关，其相关系数为0.521.研究表明，铁皮石斛4个不同极性提取物均具有较强的体外抗氧化活性，且各提取物抗氧化活性与总酚含量和总黄酮含量之间有明显的相关性，表明铁皮石斛抗氧化活性的物质基础可能就是酚类或黄酮类成分.
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