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OALib Journal期刊
ISSN: 2333-9721
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不透明红球菌转化合成α-酮异己酸的培养条件优化
DOI: 10.3724/SP.J.1145.2011.00553, PP. 553-557
Keywords: α-酮异己酸,不透明红球菌,plackett-burman法,响应面法,统计学分析,条件优化
Abstract:
利用基于非完全平衡块原理的plackett-burman法和响应面法对不透明红球菌（rhodococcusopacusdsm43250）转化合成α-酮异己酸（kic）的培养条件进行优化，以提高kic的产量.首先采用plackett-burman（pb）设计对影响kic产量的6个因素的效应进行评价，筛选出具有显著影响的关键因素――接种量、装液量和初始ph，然后通过最陡爬坡实验和box-behnken实验设计对关键因素的最佳水平范围进行研究，并利用sas软件回归分析建立了二次多项式模型，通过模型求解确定最佳培养条件为：接种量6.93%，装液量33.38ml，初始ph7.6.在优化后的培养条件下，kic的理论最高产量为31.08mg/l，在验证实验中kic最高产量为30.97mg/l，比初始产量23.93mg/l提高了29.42%，为进一步的发酵放大奠定了基础.图3表7参16
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