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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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pcr-dgge技术解析针叶和阔叶凋落物混合分解对土壤微生物群落结构的影响
DOI: 10.3724/SP.J.1145.2011.00145, PP. 145-150
Keywords: 森林凋落物,混合分解,土壤微生物,群落结构,16srdna,18srdna,多样性,dgge
Abstract:
为深入理解凋落物类型和微生物群落之间的相互关系，通过小盆+凋落袋控制实验，运用pcr-dgge技术解析了南方红壤丘陵区典型针叶树种马尾松和湿地松的凋落物分别与白栎、青冈两个阔叶树种凋落物混合分解对土壤微生物基因型多样性的影响.结果表明：1）针叶凋落物中加入阔叶后细菌群落结构未发生显著变化，将所有处理归为一类的相似度达72%，其差异仅表现在马尾松+白栎和湿地松+青冈处理土壤微生物群落16srdna的丰富度和多样性分别显著高于单一马尾松和湿地松；2）针叶凋落物中加入阔叶后真菌群落结构发生了显著变化，单一针叶处理与针阔混合处理间18srdna基因泳道带型的相似度只有28%，并且单一马尾松处理土壤18srdna的丰富度和多样性显著高于马尾松+白栎和马尾松+青冈，单一湿地松处理土壤18srdna的丰富度也显著高于湿地松+白栎和湿地松+青冈，多样性显著高于湿地松+白栎处理；3）土壤真菌18srdna的丰富度与凋落物初始c含量呈显著正相关，与凋落物初始n含量呈显著负相关.针叶凋落物中引入阔叶凋落物后，增加了凋落物中n的比重，c的比重则下降，显著影响了土壤真菌群落的结构.图3表4参36
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