|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
运动发酵单胞菌共表达α-淀粉酶和葡萄糖淀粉酶发酵甘薯生产乙醇
DOI: 10.3724/SP.J.1145.2012.00785, PP. 785-790
Keywords: 运动发酵单胞菌,乙醇发酵,甘薯,α-淀粉酶,葡萄糖淀粉酶,共表达
Abstract:
运动发酵单胞菌是乙醇发酵的极佳菌种，但其所能利用的发酵底物范围狭窄，不能利用淀粉作为发酵底物.为增加其利用底物的范围使其能够水解淀粉，本研究构建了3种表达淀粉酶的运动发酵单胞菌菌株：1）zymomonasmobilis(pamye)表达α-淀粉酶；2）z.mobilis(pga)表达葡萄糖淀粉酶；3）z.mobilis(pamyga)共同表达α-淀粉酶和葡萄糖淀粉酶.dns法测定淀粉酶活显示，每种转化菌株的胞外淀粉酶活性均高于胞内，且两种淀粉酶共表达的酶活高于这两种淀粉酶单独表达的酶活之和，说明这两种淀粉酶能够协同作用降解淀粉.对于重组菌株z.mobilis(pamyga)，约59.3%的淀粉酶活性都在胞外检测到.用淀粉含量高且耐贮存的徐薯18匀浆加少量葡萄糖作为培养基直接用上述3个菌株发酵生产乙醇.结果显示，共表达α-淀粉酶和葡萄糖淀粉酶的重组菌株z.mobilis(pamyga)的乙醇产量为54.7g/l，达到了理论值的83.2%，表明本研究得到了能够直接高效利用淀粉生产乙醇的运动发酵单胞菌的菌株.图5表1参20
| [1] | 1rogerspl,jeonyj,leekj,lawfordhg.zymomonasmobilisforfuelethanolandhighervalueproducts.advbiochemengbiotechnol,2007,108:263~288
|
| [2] | 2rogerspl,leekj,tribede.kineicsofalcoholproductionbyzymomonasmobilisathighsugarconcentration.advbiochemeng,1982,23:27~84
|
| [3] | 3swingsj,leyjd.thebiologyofzymomonas.bacterialrev,1977,41(1):1~46
|
| [4] | 4baifw,andersonwa,moo-youngm.ethanolfermentationtechnologiesfromsugarandstarchfeedstocks.biotechadv,2008,26(1):89~105
|
| [5] | 5zhangl,liuq,wangq.sweetpotatoinchina.in:loebensteing,thottappillyg.thesweetpotato.thenetherland:springerdordrecht,2009.325~357
|
| [6] | 6reddyovs,basappasc.directfermentationofcassavastarchtoethanolbymixedculturesofendomycopsisfibuligeraandzymomonasmobilis:synergismandlimitations.biotechnollett,1996,18(11):1315~1318
|
| [7] | 7carlosma,guillermorc,faustinos,danleyasc.productionofamylolyticenzymesbybacillusamyloliquefaciensinpurecultureandinco-culturewithzymomonasmobilis.biotechnollett,1999,21(3):249~252
|
| [8] | 8brestic-goachetn,gunasekaranp,camib,barattij.transferandexpressionofabacilluslicheniformisα-amylasegeneinzymomonasmobilis.archmicrobiol,1990,153(3):219~225
|
| [9] | 9skotnickiml,warrrg,goodmanae,leekj,rogerspl.highproductivityethanolfermentationusingzymomonasmobilis.biochemsocsymp,1983,48:53~86
|
| [10] | 10sup,delaneysf,rogerspl.cloningandexpressionofaβ-glucosidasegenefromxanthomonasalbilineansinescherichiacoliandzymomonasmobilis.jbiotechnol,1989,9(2):139~152
|
| [11] | 11hemx,fengh,baif,liy,liux,zhangyz.directproductionofethanolfromrawsweetpotatostarchusinggeneticallyengineeredzymomonasmobilis.afrjmicrobiolres,2009,3(11):721~726
|
| [12] | 12kovachme,elzerph,hillds,robertsongt,farrisma,rooprm,petersonkm.fournewderivativesofthebroad-host-rangecloningvectorpbbr1mcs,carryingdifferentantibiotic-resistancecassettes.gene,1995,166(1):175~176
|
| [13] | 13wugf(吴国峰),ligq(李国全),mayq(马永强).工业发酵分析.beijing:chinalightindustrypress(北京:中国轻工业出版社),1980
|
| [14] | 14conwayt,osmanya,konnanji,hoffmannem,ingramlo.promoterandnucleotidesequencesofthezymomonasmobilispyruvatedecarboxylase.jbacteriol,1987,169(3):949~954
|
| [15] | 15lingerjg,adneyws,darzinsa.heterologousexpressionandextracellularsecretionofcellulolyticenzymesbyzymomonasmobilis.applenvironmicrobiol,2010,76(19):6360~6369
|
| [16] | 16sandkvistm.biologyoftypeiisecretion.molmicrobiol,2001,40(2):271~283
|
| [17] | 17fujiim,hommat,taniguchim.synergismof-amylaseandglucoamylaseonhydrolysisofnativestarchgranules.biotechnolbioeng,1988,32(7):910~915
|
| [18] | 18taox(陶向),zhangyw(张勇为),jiangys(姜玉松),wanghy(王海燕),zhangyz(张义正).changesinrootstarchcontentsofsweetpotatocultivarsduringstorage.chinjapplenvironbiol(应用与环境生物学报),2010,16(5):741~744
|
| [19] | 19aggarwalnk,nigamp,singhd,yadavbs.processoptimizationfortheproductionofsugarforthebioethanolindustryfromsorghum,anon-conventionalsourceofstarch.worldjmicrobiolbiotechnol,2001,17(4):411~415
|
| [20] | 20palmqviste,hahn-hägerdalb.fermentationoflignocellulosichydrolysatesii:inhibitorsandmechanismsofinhibition.bioresourtechnol,2000,74(1):25~33
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
