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OALib Journal期刊
ISSN: 2333-9721
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林木外生菌根真菌彩色豆马勃巢式pcr检测
DOI: 10.3724/SP.J.1145.2012.00298, PP. 298-302
Keywords: 外生菌根真菌,彩色豆马勃,巢式pcr,分子检测
Abstract:
为探讨林木外生菌根真菌的分子检测方法，利用真菌通用引物its1-f/its4-b扩增了外生菌根真菌彩色豆马勃的核糖体dna内转录间隔区并进行了序列测定.通过序列比较，设计了一对彩色豆马勃特异性引物ptf/ptr.利用该对特异性引物与its1-f/its4-b组合进行巢式pcr，能从供试的彩色豆马勃10个菌株中特异性地扩增出1条347bp的条带，而供试的其他6个参比菌株未出现扩增产物.经分析，该巢式pcr检测技术的灵敏度可达到10fg的dna，是常规pcr检测的1000倍.利用该技术从马尾松苗木菌根中检测到目的外生菌根真菌.这表明采用本研究设计的特异性引物，利用巢式pcr技术可以灵敏、准确地从林木外生菌根中检测出彩色豆马勃.图4参20
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