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OALib Journal期刊
ISSN: 2333-9721
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促进氰钴胺素转化为腺苷钴胺素的基因表达与鉴定
DOI: 10.3724/SP.J.1145.2012.00267, PP. 267-271
Keywords: 甘油,钴胺素,辅酶b12,腺苷转移酶,克雷伯氏杆菌,重组菌
Abstract:
辅酶b12作为甘油脱水酶（gdht）的辅酶参与到klebsiellapneumoniae代谢甘油生成1,3-丙二醇（1,3-pd）和3-羟基丙酸（3-hp）的途径中.前期研究表明底物甘油可以导致辅酶b12分子中co―c键的断裂，进而引起gdht的失活.为进一步研究非活性的维生素b12（cncbi）转化为具有活性的辅酶b12（adocbi）的过程，从k.pneumoniae中克隆得到了atp:钴（i）胺素腺苷转移酶（aca）基因btur和还原酶基因ycik，并成功构建了双启动子表达质粒puc18-tac-btur-tac-ycik，将表达质粒转化escherichiacolijm109获得了一株重组菌.利用改进的分析方法，结果表明：1）重组蛋白具有腺苷转移酶的活性；2）重组菌可以很好地将非活性的钴胺素，如维生素b12，转化生成具有活性的辅酶b12.图9表1参16？
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