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OALib Journal期刊
ISSN: 2333-9721
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锌对汞胁迫下小麦幼苗生理特性的影响
DOI: 10.3724/SP.J.1145.2012.00212, PP. 212-217
Keywords: 锌,汞胁迫,小麦,生理特性,抗氧化酶
Abstract:
通过沙基培养方法研究不同浓度锌（zn）对汞（hg）胁迫下小麦幼苗叶绿素相对含量（spad）、丙二醛（mda）与脯氨酸的积累及抗氧化酶活性等的影响.结果表明：单独hg（10mgl-1）胁迫下，小麦幼苗叶绿素含量降低，超氧化物歧化酶（sod）与过氧化氢酶（cat）活性受到抑制，mda与脯氨酸含量升高.加入10mgl-1zn后，叶绿素含量比hg单独胁迫升高了5.6%，mda含量降低了32.4%，表明该浓度zn在一定程度上缓解了hg对小麦幼苗的毒害；zn浓度高于10mgl-1的处理中，叶绿素含量逐渐降低，mda则呈升高趋势，表明随zn浓度升高，zn对hg毒害的缓解作用逐渐降低.低浓度zn（10~20mgl-1）提高了hg胁迫下小麦幼苗sod与cat活性，其活性随zn浓度升高逐渐增加，20mgl-1zn处理下，两种酶活性比hg单独胁迫分别升高了103.3%与71.0%，高浓度zn（50~100mgl-1）处理下，两种酶活性则呈下降趋势，表明zn对hg胁迫下两种酶活性的促进作用逐渐减弱.在试验设置的zn浓度范围内，脯氨酸含量均低于hg单独胁迫，并且低于对照，说明外加zn抑制了脯氨酸的生成.综上所述，10~20mgl-1的zn可以在一定程度上缓解10mgl-1hg对小麦幼苗造成的毒害.图5参34
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