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OALib Journal期刊
ISSN: 2333-9721
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环孢霉素a对nit-1胰岛β细胞基因表达谱的影响
, PP. 853-857
Keywords: 环孢霉素a,nit-1细胞,胰岛β细胞,rt-pcr,基因芯片
Abstract:
目的采用基因芯片技术,观察免疫抑制剂环孢霉素a(csa)对nit-1胰岛β细胞基因表达谱的影响。方法体外培养nit-1胰岛β细胞,以10μmol/lcsa处理24h。应用基因芯片分别检测csa处理24h及空白对照组nit-1胰岛β细胞的基因表达谱。结果csa作用于nit-1胰岛β细胞24h后,在4096条基因中,有38条基因表达上调,其中已知功能基因13条,主要是与应激反应、蛋白质合成及细胞生长相关的基因。有46条基因表达下调,其中已知功能基因25条,主要是与细胞生长发育、氧化磷酸化过程及蛋白合成有关的基因。rt-pcr技术验证了zfr、tpi和pax63个基因表达的变化。结论csa作用于nit-1胰岛β细胞24h后,可影响nit-1细胞中多种基因的表达,其中对细胞生长发育、氧化磷酸化等有关基因表达的抑制作用,可能与csa抑制nit-1细胞胰岛素释放的作用机制相关。本研究为进一步深入研究csa对胰岛β细胞的作用机制提供了线索和依据。
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