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OALib Journal期刊
ISSN: 2333-9721
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尾加压素刺激血管内皮细胞分泌肾上腺髓质素的机制
, PP. 791-793
Keywords: 内皮细胞,尾加压素,肾上腺髓质素,信号转导
Abstract:
目的研究人尾加压素(uⅱ)刺激人血管内皮细胞(hvec)分泌肾上腺髓质素(adm)的机制。方法以不同浓度的uⅱ(10-10、10-9、10-8、10-7mol/l)刺激培养的hvec,放射免疫法测定其分泌adm的量,以及不同的细胞信号转导阻滞剂(丝裂素活化蛋白激酶阻断剂pd98059、钙调素依赖性蛋白激酶阻断剂w7、p38蛋白激酶抑制剂sb202190、钙通道阻断剂nicardipine、蛋白激酶c阻断剂h7及钙调神经磷酸酶抑制剂环孢霉素)对其分泌的影响。结果uⅱ可促进hvec分泌adm,且呈时间和浓度依赖性。各组与对照组之间有显著差别。pd98059、w7、sb202190及nicardipine均能抑制uⅱ刺激的hvec对adm的分泌,其抑制率分别为67%(p<0.01)、77%(p<0.01)、23%(p<0.05)、24%(p<0.05)。而h7、csa对uⅱ刺激hvec分泌adm无显著影响(p>0.05)。结论uⅱ能刺激hvec分泌adm,其机制与ca2+、mapk、campk及p38信号转导通路介导有关。
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