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OALib Journal期刊
ISSN: 2333-9721
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血管紧张素-(1-7)对氧化低密度脂蛋白诱导人血管内皮细胞纤溶功能异常的调节
, PP. 633-637
Keywords: 血管紧张素-(1-7),组织纤溶酶原激活物,组织纤溶酶原激活物抑制物-1,氧化低密度脂蛋白,人脐静脉内皮细胞
Abstract:
目的探讨血管紧张素-(1-7)[ang-(1-7)]对氧化低密度脂蛋白(ox-ldl)诱导血管内皮细胞分泌组织型纤溶酶原激活物(t-pa)及其抑制物-1(pai-1)的影响。方法用酶消化法收集并培养人脐静脉内皮细胞(huvecs),分别以不同终浓度的ang-(1-7)(10、100、1000nmol/l)和ox-ldl[25、50、100mg/l(蛋白质含量)]进行单独或联合刺激,并以a-779(终浓度为100nmol/l)进行干预,孵育24h。以elisa方法检测培养基上清液中t-pa和pai-1蛋白含量,rt-pcr法检测pai-1和t-pamrna的表达。结果ox-ldl使pai-1的分泌量显著增加,t-pa分泌显著降低,pai-1mrna表达升高,t-pamrna表达降低(p<0.001~0.05),并呈剂量依赖性;ang-(1-7)使pai-1分泌量及pai-1mrna表达显著降低,亦呈剂量依赖性(p<0.01~0.05),而对t-pa分泌及t-pamrna表达无影响;在混合刺激组中,与ox-ldl组比较,ang-(1-7)使pai-1及pai-1mrna表达降低、t-pa及t-pamrna表达升高(p<0.01~0.05),但pai-1及pai-1mrna表达仍高于对照组,t-pa及t-pamrna表达仍低于对照组(p<0.05)。加入a-779后,ang-(1-7)的作用消失。结论ang-(1-7)对氧化低密度脂蛋白诱导的血管内皮细胞纤溶异常具有显著的调节作用,此作用是通过特异性受体介导的。
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