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OALib Journal期刊
ISSN: 2333-9721
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腺病毒载体介导的vegf基因转染对c17.2神经干细胞凋亡的影响
, PP. 366-370
Keywords: 神经干细胞,血管内皮生长因子,细胞凋亡,腺病毒载体,缺氧
Abstract:
目的探讨重组腺病毒介导血管内皮生长因子(vegf)165基因治疗对缺氧状态下对神经干细胞凋亡的作用。方法体外培养c17.2神经干细胞,建立神经干细胞缺氧模型。将携带人类vegf基因的重组腺病毒感染c17.2神经干细胞,western-blotting分析vegf蛋白的表达,并用流式细胞术测定细胞凋亡率的变化。hoechst33342染色荧光显微镜观察凋亡小体。结果转染padcmvvegf165的c17.2神经干细胞成功表达vegf蛋白;缺氧后神经干细胞凋亡率为(19.98±0.55)%,而转染padcmvvegf165后的细胞凋亡率为(10.38±0.48)%(p<0.01),转染padcmvvegf165+vegf反义寡核脱氧核酸经干细胞凋亡率为(19.07±0.64)%,与对照组无显著差异(p>0.05)。结论腺病毒介导的vegf165基因能高效表达vegf;外源性vegf对c17.2神经干细胞具有抗凋亡作用,能够提高c17.2神经干细胞对缺氧的耐受性,有利于神经干细胞的生存。
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