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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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人巨噬细胞源性泡沫细胞分化中kir2.1通道的表达
, PP. 1461-1467
Keywords: 离子通道,胆固醇代谢,细胞分化,动脉粥样硬化
Abstract:
目的kir2.1通道是控制骨髓源性巨噬细胞增殖、活化和凋亡的重要离子通道之一,并且还参与细胞分化。本研究观察kir2.1通道mrna及蛋白在人单核细胞源性巨噬细胞向泡沫细胞分化过程中的表达。方法采用密度梯度离心加贴壁黏附法,从男性健康志愿者的外周血中分离单核细胞,经5d培养后分化为巨噬细胞。在建立人巨噬细胞源性泡沫细胞模型的基础上,采用rt-pcr,蛋白质印迹及免疫细胞化学方法研究kir2.1通道的表达。结果将巨噬细胞同30mg/l氧化修饰低密度脂蛋白(oxldl)于37℃孵育60h后,细胞体积增大,并有许多红色的脂质颗粒沉积于细胞质内,细胞内的总胆固醇(tc)、游离胆固醇(fc)及胆固醇酯(ce)的含量分别从(54.79±28.304)mg/g、(47.968±26.787)mg/g和(6.822±3.437)mg/g增加至(229.775±57.453)mg/g、(96.241±24.003)mg/g和(133.535±36.292)mg/g,ce/tc从(14.437±6.781)%提高到[(57.946±3.507)%(n=7,p<0.05)]。但是,kir2.1通道mrna的扩增量在巨噬细胞和分化的泡沫细胞之间无显著差异[(59.074±10.566)%vs(46.98±12.527)%,n=5,p＞0.05];同样,kir2.1通道蛋白在巨噬细胞和分化的泡沫细胞之间亦无显著差异[(60.527±18.621)%vs(50.243±11.583)%,n=6,p＞0.05]。结论人单核细胞源性巨噬细胞同30mg/loxldl孵育60h后可分化为泡沫细胞,但kir2.1通道的表达无明显改变。
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