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OALib Journal期刊
ISSN: 2333-9721
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植物内生细菌xg32菌株16srrna寡核苷酸探针杂交条件优化和特异性验证
, PP. 89-95
Keywords: 荧光原位杂交,杂交条件优化,特异探针验证
Abstract:
为了能利用荧光原位杂交方法来研究植物内生细菌xg32菌株在植物体内的定殖和分布,利用xg32菌株的16srrna中段特异性序列和genbank数据库,用blast、dnaclub和oligo软件设计了一个在理论上具有特异性的探针,在优化该探针的杂交条件基础上,通过与12个亲缘关系相邻和相近的菌株反复杂交来验证探针的特异性.结果显示,xg32探针适宜的杂交条件为:杂交缓冲液中甲酰胺浓度为35%,nacl浓度为1.2mol/l,杂交温度为42℃,清洗缓冲液中nacl浓度为0.02mol/l;探针与xg3探针.
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