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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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聚苯乙烯微球中荧光共振能量转移的研究
DOI: 10.3969/j.jssn.1000-2006.2011.02.018, PP. 83-87
Keywords: 聚苯乙烯,荧光微球,分散聚合,荧光共振能量转移
Abstract:
采用分散共聚合法制备了单分散的负载有1,8-萘酰亚胺染料的荧光微球。利用供体和受体染料间的荧光共振能量转移，荧光微球在激发供体染料时同时实现了供体和受体染料的双重荧光发射信号。结果表明：当微球中染料的浓度相对较低（质量分数小于0？2%）时，荧光共振能量就可以有效地发生转移。聚合过程中增加供体或受体染料的浓度都会提高能量转移效率，这是浓度增加使得染料分子间距离缩短造成的。通过调整微球中染料浓度可以获得不同能量转移效率、具有荧光编码信号的系列荧光微球。这类粒径均一的荧光微球在多元生物分析中有潜在的应用价值。
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