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OALib Journal期刊
ISSN: 2333-9721
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韩国伞滑刃属线虫的形态和分子特征
DOI: 10.3969/j.jssn.1000-2006.2011.01.033, PP. 142-142
Keywords: 伞滑刃属线虫属,测序,分子特征,鉴定
Abstract:
以锥尾伞滑刃线虫（bursaphelenchusconicaudatus）作为对照，在韩国不同地域和寄主中采集了15个松材线虫分离物、3个拟松材线虫的分离物及5个未鉴定的该属的分离物,并对其进行了its和d2d3rdna序列分析。以单条雌线虫的dna为模板,its和d2d3区由pcr仪扩增并克隆其序列。结果表明,所有松材线虫的its和d2d3区序列相同，没有种内变异。然而2个采自黑松和红松的拟松材线虫分离物的基因型分别是亚洲型和欧洲型。5个未鉴定线虫的数据表明，它们分别接近b.tusciae、b.lini、b.thailandae、b.doui和b.hylobianum,该结果同时被形态学结果所支持。利用5种酶（hinfi、alui、mspi、haeiii、rsa）,pcr产物克隆并测序的数据也可以区分不同种和基因型。
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