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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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杉木srap-pcr体系优化
DOI: 10.3969/j.issn.1000-2006.2014.01.003, PP. 15-20
Keywords: 杉木,srap-pcr,体系优化
Abstract:
为建立杉木srap-pcr反应体系,利用l16(45)正交设计对影响杉木srap-pcr反应体系的mg2+、dntps、引物、taq酶和dna浓度5种因素的4个水平进行优化实验,结合正交直观分析和方差分析,对影响反应较大的mg2+、dntps和引物浓度进行单因素实验,最终确定杉木srap-pcr最佳的反应体系为:在20μl的pcr反应体系中,mg2+浓度为2.25mmol/l、dntps为0.15mmol/l、引物浓度为0.4μmol/l、taq酶为1.5μmol/min、模板dna为60ng,10×pcrbuffer2μl,不足部分用双蒸水补充至20μl。pcr反应程序的两步最适退火温度第1步为35℃,第2步为53℃。利用上述反应体系进行杉木pcr扩增,能得到清晰、稳定的条带。
| [1] | 郑仁华,施季森,翁玉榛,等.福建省杉木育种战略研究[j].林业科技开发,2008,22(2):1-6.zhengrh,shijs,wengyz,etal.thereachofcunninghamialanceolatabreedingmeasuresinfujianprovince[j].chinaforestryscienceandtechnology,2008,22(2):1-6.
|
| [2] | 何祯祥,施季森,王明庥,等.杉木杂种群体分子框架遗传连锁图初报[j].南京林业大学学报,2000,24(6):22-26.hezx,shijs,wangmx,etal.theconstructionofmolecularlinkageframemapinchinesefir[j].journalofnanjingforestryuniversity,2000,24(6):22-26.
|
| [3] | sunsj,gaow,linsq,etal.analysisofgeneticdiversityinganodermapopulationwithanovelmolecularmarkersrap[j].appliedmicrobiologyandbiotechnology,2006,72(3):537-543.
|
| [4] | 徐操,赵宝华.srap分子标记的研究进展及其应用[j].生命科学仪器,2009,7(4):24-27.xuc,zhaobh.thedevelopmentandapplicationofsrapmolecularmarkers[j].lifescienceinstruments,2009,7(4):24-27.
|
| [5] | 孙佳琦,梁建国,石少川,等.srap标记在观赏植物遗传育种中的应用[j].分子植物育种,2010,8(3):577-588.sunjq,liangjg,shisc,etal.applicationsofsrapmarkeringeneticsandbreedingofornamentalplants[j].molecularplantbreeding,2010,8(3):577-588.
|
| [6] | 吴莺莺,彭方仁,郝明灼,等.油茶srap-pcr反应体系的建立[j].南京林业大学学报:自然科学版,2011,35(5):112-116.wuyy,pengfr,haomz,etal.establishmentreactionsystemofthesrap-pcrincamelliaoleifera[j].journalofnanjingforestryuniversity:naturalsciencesedition,2011,35(5):112-116.
|
| [7] | 李慧芝,尹燕枰,张春庆,等.srap在葱栽培品种遗传多样性研究中的适用性分析[j].园艺学报,2007,34(4):929-934.lihz,yinyp,zhangcq,etal.evaluationofapplicationofsraponanalysisofgeneticdiversityincultivarsofalliumfistulosuml.[j].actahorticulturaesinica,2007,34(4):929-934.
|
| [8] | 井赵斌,俞靓,魏琳,等.本氏针茅srap-pcr反应体系的建立及引物筛选[j].草业科学,2012,29(2):219-228.jingzb,yuj,weil,etal.establishmentofsrap-pcrsystemandprimersscreeningofstipabungeana[j].prataculturalscience,2012,29(2):219-228.
|
| [9] | 姜艳,刘进平.胡椒srap反应体系的建立和优化[j].中国农学通报,2012,28(31):141-145.jiangy,liujp.establishmentandoptimizationofblackpeppersrapreactionsystem[j].chineseagriculturalsciencebulletin,2012,28(31):141-145.
|
| [10] | wangg,panj,lix,etal.constructionofacucumbergeneticlinkagemapwithsrapmarkersandlocationofthegenesforlateralbranchtraits[j].scienceinchinaseriesc:lifesciences,2005,48(3):213-220.
|
| [11] | 姜树坤,马慧,刘君,等.利用srap标记分析玉米遗传多样性[j].分子植物育种,2007,5(3):412-416.jiangsk,mah,liuj,etal.geneticdiversityinmaizeinbredlinesrevealedbysrapsmarker[j].molecularplantbreeding,2007,5(3):412-416.
|
| [12] | 于永福.杉科植物的起源、演化及其分布[j].植物分类学报,1995,33(4):362-389.yuyf.origin,evolutionanddistributionofthetaxodiaceae[j].actaphytotaxonomicasinica,1995,33(4):362-389.
|
| [13] | 侯振宏,张小全,徐德应,等.杉木人工林生物量和生产力研究[j].中国农学通报,2009,25(5):97-103.houzh,zhangxq,xudy,etal.studyonbiomassandproductivityofchinesefirplantation[j].chineseagriculturalsciencebulletin,2009,25(5):97-103.
|
| [14] | 郑仁华,施季森.福建省杉木良种繁育现状与对策[j].林业科技开发,2004,18(2):3-7.zhengrh,shijs.thepresentsituationandthecountermeasuresofcunninghamialanceolatabreedinginfujianprovince[j].chinaforestryscienceandtechnology,2004,18(2):3-7.
|
| [15] | 柳李旺,龚义勤,黄浩,等.新型分子标记――srap与trap及其应用[j].遗传,2004,26(5):777-781.liulw,gongyq,huangh,etal.novelmolecularmarkersystems―srapandtrapandtheirapplication[j].hereditas,2004,26(5):777-781.
|
| [16] | lig,quiroscf.sequence-relatedamplifiedpolymorphism(srap),anewmarkersystembasedonasimplepcrreaction:itsapplicationtomappingandgenetagginginbrassica[j].theoreticalandappliedgenetics,2001,103(2):455-461.
|
| [17] | 邱帅,丁信誉,席梦利,等.东方百合srap体系优化及引物筛选[j].南京林业大学学报:自然科学版,2013,37(3):6-10.qius,dingxy,ximl,etal.optimizationofsrap-pcrsystemandprimersscreeningoforientallilyhybrids[j].journalofnanjingforestryuniversity:naturalsciencesedition,2013,37(3):6-10.
|
| [18] | 李建军,刘志坚,肖层林,等.srap技术在遗传的研究进展[j].现代生物医学进展,2007,7(5):783-786.lijj,liuzj,xiaocl,etal.researchprogressonsraptechniqueingenetics[j].progressinmodernbiomedicine,2007,7(5);783-786.
|
| [19] | 丁锦平,高国庆,周瑞阳,等.花生srap反应体系的建立与优化[j].安徽农业科学,2008,36(10):4009-4010.dingjp,gaogq,zhoury,etal.establishmentandoptimizationofsrapreactionconditionsforarachishypogaeal.[j].journalofanhuiagriculturalsciences,2008,36(10):4009-4010.
|
| [20] | 张大伟,马林,陈全家,等.棉花srap反应体系的建立与优化[j].新疆农业大学学报,2008,31(5):23-27.zhangdw,mal,chenqj,etal.establishmentandoptimizationofreactionsystemofsequence-relatedamplifiedpolymorphismofcotton[j].journalofxinjiangagriculturaluniversity,2008,31(5):23-27.
|
| [21] | elizabetháclaxtone.synthesisofallenesby2,3-sigmatropicrearrangementofprop-2-yn-1-yloxoniumylidesformedinrhodium(ii)carboxylatecatalyzed-reactionsofdiazo-compounds[j].jchemsoc-chemcommun,1990(1):46-48.
|
| [22] | 何正文,刘运生,陈立华,等.正交设计直观分析法优化pcr条件[j].湖南医科大学学报,1998,23(4):76-77.hezw,liuys,chenlh,etal.optimizationofpcrconditionsdirectanalysisbasedonorthogonaldesign[j].bulletinofhunanmedicaluniversity,1988,23(4):76-77.
|
| [23] | 朱红兵,席凯强.spss17.0中的正交试验设计与数据分析[j].首都体育学院学报,2013,25(3):283-288.zhuhb,xikq.theorthogonalexperimentaldesigninspss17.0anddataanalysis[j].journalofcapitaluniversityofphysicaleducationandsports,2013,25(3):283-288.
|
| [24] | 盛文涛,周劲松,汤泳萍,等.芦笋srap反应体系优化及引物筛选[j].分子植物育种,2010,8(3):612-618.shengwt,zhoujs,tangyp,etal.optimizationofsrapreactionsystemandselectionofprimersforasparagusofficinalisl.[j].molecularplantbreeding,2010,8(3):612-618.
|
| [25] | 邹明宏,郭凌飞,曾辉,等.山竹子基因组dna提取及srap反应体系优化[j].经济林研究,2009,27(3):38-41.zoumh,guolf,zengh,etal.genomednaextractionandsrapreactionsystemoptimizationofgarciniamangostana[j].nonwoodforestresearch,2009,27(3):38-41.
|
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